SBB Certification Final Exam Questions
and Correct Answers
Identifying information on the donor form should include: - CORRECT ANSWER-Date
and time of donation
Name
Address
Telephone #
Gender
Age or DOB
A record of reasons for prior deferrals, if any
Donors must be given educational material regarding: - CORRECT ANSWER-Infection
diseases transmitted by blood transfusion
Signs and symptoms of AIDS
Risks of procedure
Circumstances in which infection disease testing is not performed
should be given chance to ask questions
Donor qualifications: pay particular attention to the following: - CORRECT ANSWER-
Donation interval
vitals
HGB/HCT
receipt of blood or other human tissue
Immunizations
travel
infectious diseases
Leukapheresis donors may be given what drugs to enhance collections? - CORRECT
ANSWER-Hydroxyethyl starch
Corticosteroids
Growth factors
donor tubes from each donation are tested for what? - CORRECT ANSWER-
ABO/RH/ABSC
HBsAg
anti-HBc
Anti-HCV
HCV RNA
Anti-HIV-1/2
HIV-1 RNA
Anti-HTLV-I/II
Syphilis
,ALT is not required by FDA or AABB
Plasma manufacturers can use donors positive for what? - CORRECT ANSWER-Anti-
HBc
Anti-HTLV-I/II
Cytapheresis and Auto donors must be tested for ID how often - CORRECT ANSWER-
Every 30 days
reactive ID results must be... - CORRECT ANSWER-tested in duplicate for confirmation
Neutralization: HBsAg and HIV-1 Ag
Western Blot - Anti-HIV
RIBA - Anti-HCV
Anti-HTLV confirmed by repeating the test using another manufacturers EIA test
HTLV testing and deferral - CORRECT ANSWER-Two+ positives: 2 donations by same
manufacturer = indefinite deferral
1+ by one manuf. and 1+ by another manuf = indef.defer.
1+ by one manuf. and negative by another manuf. = 8 week deferral
What does Dextrose in additive do for cells? - CORRECT ANSWER-supports ATP
generation
What does Adenine in additive do for cells? - CORRECT ANSWER-provides substrate
for ATP synthesis
What does sodium biphosphate in additive do for cells? - CORRECT ANSWER-controls
pH
What does citrate in additive do for cells? - CORRECT ANSWER-prevents coagulation
What is the storage time if additive used is CPD, CP2D? - CORRECT ANSWER-21
days
What is the storage time if additive used is CPDA-1? - CORRECT ANSWER-35 days
What is the storage time if additive used is AS-1 (Adsol)? - CORRECT ANSWER-42
days
What is the storage time if additive used is AS-3 (nutricel)? - CORRECT ANSWER-42
days
What is the storage time if additive used is AS-5 (Optisol)? - CORRECT ANSWER-42
days
Whole Blood storage temp and description - CORRECT ANSWER-1-6C
, contains all blood elements plus the anticoagulant-preservative commonly used as a
source for component production
RBC storage and description - CORRECT ANSWER-1-6C
Whole blood with plasma removed
RBCs without additives must have final hct <80%
Frozen RBC storage and description - CORRECT ANSWER--65C or colder
frozen within 6 days if no additive solution
RBC w/additive must be frozen before expiration
must be put in freezer within 4 hours of removed from 4C
Rejuvenated RBC description - CORRECT ANSWER-rejuvenation should restore 2,3-
DPG to 75% of normal level and ATP above normal levels.
If done before 14 days may cause high 2,3-DPG which impairs O2 up-take.
AS-3 and AS-5 cannot be rejuvenated
Deglyced RBCs storage and description - CORRECT ANSWER-37C warmer or
waterbath (20-25 minutes)
washed with solutions of decreasing osmolarity to avoid hemolysis.
must remove cryoprotectant agents, minimial free hgb, and result in recovery of at least
80% RBC volume.
open system:1-6C with 24 hr expiration.
closed system: 14 days or as FDA approved
What should the osmolality of a deglyced unit be? - CORRECT ANSWER-<500.
Osmolality is a fxn of free glycerol therefore an increase is a result of improper
deglycing
What should free hgb in last wash be from decglyed unit? - CORRECT ANSWER-<200
Washed RBCs storage - CORRECT ANSWER-1-6C
washed with sterile normal saline to remove most of plasma
24 hour expiration
FFP storage and description - CORRECT ANSWER-prepared from WB or collected
using apheresis.
frozen at -18C or below
contains max levels of labile or nonlabile coag factors
Thaw between 30-37C
Thawed plasma - CORRECT ANSWER-FFP thawed but not transfused within 24 hrs
can be stored 1-6C for 5 days
reduction in Factors V and VII
and Correct Answers
Identifying information on the donor form should include: - CORRECT ANSWER-Date
and time of donation
Name
Address
Telephone #
Gender
Age or DOB
A record of reasons for prior deferrals, if any
Donors must be given educational material regarding: - CORRECT ANSWER-Infection
diseases transmitted by blood transfusion
Signs and symptoms of AIDS
Risks of procedure
Circumstances in which infection disease testing is not performed
should be given chance to ask questions
Donor qualifications: pay particular attention to the following: - CORRECT ANSWER-
Donation interval
vitals
HGB/HCT
receipt of blood or other human tissue
Immunizations
travel
infectious diseases
Leukapheresis donors may be given what drugs to enhance collections? - CORRECT
ANSWER-Hydroxyethyl starch
Corticosteroids
Growth factors
donor tubes from each donation are tested for what? - CORRECT ANSWER-
ABO/RH/ABSC
HBsAg
anti-HBc
Anti-HCV
HCV RNA
Anti-HIV-1/2
HIV-1 RNA
Anti-HTLV-I/II
Syphilis
,ALT is not required by FDA or AABB
Plasma manufacturers can use donors positive for what? - CORRECT ANSWER-Anti-
HBc
Anti-HTLV-I/II
Cytapheresis and Auto donors must be tested for ID how often - CORRECT ANSWER-
Every 30 days
reactive ID results must be... - CORRECT ANSWER-tested in duplicate for confirmation
Neutralization: HBsAg and HIV-1 Ag
Western Blot - Anti-HIV
RIBA - Anti-HCV
Anti-HTLV confirmed by repeating the test using another manufacturers EIA test
HTLV testing and deferral - CORRECT ANSWER-Two+ positives: 2 donations by same
manufacturer = indefinite deferral
1+ by one manuf. and 1+ by another manuf = indef.defer.
1+ by one manuf. and negative by another manuf. = 8 week deferral
What does Dextrose in additive do for cells? - CORRECT ANSWER-supports ATP
generation
What does Adenine in additive do for cells? - CORRECT ANSWER-provides substrate
for ATP synthesis
What does sodium biphosphate in additive do for cells? - CORRECT ANSWER-controls
pH
What does citrate in additive do for cells? - CORRECT ANSWER-prevents coagulation
What is the storage time if additive used is CPD, CP2D? - CORRECT ANSWER-21
days
What is the storage time if additive used is CPDA-1? - CORRECT ANSWER-35 days
What is the storage time if additive used is AS-1 (Adsol)? - CORRECT ANSWER-42
days
What is the storage time if additive used is AS-3 (nutricel)? - CORRECT ANSWER-42
days
What is the storage time if additive used is AS-5 (Optisol)? - CORRECT ANSWER-42
days
Whole Blood storage temp and description - CORRECT ANSWER-1-6C
, contains all blood elements plus the anticoagulant-preservative commonly used as a
source for component production
RBC storage and description - CORRECT ANSWER-1-6C
Whole blood with plasma removed
RBCs without additives must have final hct <80%
Frozen RBC storage and description - CORRECT ANSWER--65C or colder
frozen within 6 days if no additive solution
RBC w/additive must be frozen before expiration
must be put in freezer within 4 hours of removed from 4C
Rejuvenated RBC description - CORRECT ANSWER-rejuvenation should restore 2,3-
DPG to 75% of normal level and ATP above normal levels.
If done before 14 days may cause high 2,3-DPG which impairs O2 up-take.
AS-3 and AS-5 cannot be rejuvenated
Deglyced RBCs storage and description - CORRECT ANSWER-37C warmer or
waterbath (20-25 minutes)
washed with solutions of decreasing osmolarity to avoid hemolysis.
must remove cryoprotectant agents, minimial free hgb, and result in recovery of at least
80% RBC volume.
open system:1-6C with 24 hr expiration.
closed system: 14 days or as FDA approved
What should the osmolality of a deglyced unit be? - CORRECT ANSWER-<500.
Osmolality is a fxn of free glycerol therefore an increase is a result of improper
deglycing
What should free hgb in last wash be from decglyed unit? - CORRECT ANSWER-<200
Washed RBCs storage - CORRECT ANSWER-1-6C
washed with sterile normal saline to remove most of plasma
24 hour expiration
FFP storage and description - CORRECT ANSWER-prepared from WB or collected
using apheresis.
frozen at -18C or below
contains max levels of labile or nonlabile coag factors
Thaw between 30-37C
Thawed plasma - CORRECT ANSWER-FFP thawed but not transfused within 24 hrs
can be stored 1-6C for 5 days
reduction in Factors V and VII
