with complete solutions
Exp 1.1 - Lab Safety + Exposure to Microbes - ANSWER-
Must follow safety rules because? - ANSWER-microbes are not visible to naked eye.
Aseptic Technique? - ANSWER-"Contamination Free" Technique
-reduces spreading microbes to self + others
-use cuz results from contaminated samples are hard to interpret
General Lab Safety Rules? - ANSWER--do NOT enter lab w/o permission
-instructor must be in lab @ all times
-pay close attention to what you're doing @ all times
-treat all microorganisms used as if they're dangerous
-disinfect lab benches before/after each lab period
-wash hand before/after each lab period
-keep doors closed during experiments
-know locations of safety equipment (Ie. Fire extinguisher, fire blanket, safety shower,
eyewasher)
-ask about unclear instructions
-only perform exp's authorized by TA instructor
Rules BEFORE lab starts? - ANSWER--Books, jackets, backpacks --> on shelves
-Lab coat
-Wash hands
-Disinfect lab bench
Rules AFTER lab ends? - ANSWER--Alcohol burner flame OFF
-Lab coat off
-Wash hands
-Disinfect lab bench
-Take all belongings
Appropriate Lab Behavior? - ANSWER--Dress Code: Closed toe shoes, Long pants
(must change + tardy if dressed improperly), Secure loose clothing, Tie hair up.
-Safety: Wear + button up lab coats, Nitrile gloves, Safety glasses
-Careful with alcohol burners: Never leave lit burner unattended, Keep ethanol
(flammable) away from burners.