MICROBIOLOGY THEORY AND
APPLICATION TEST 2 WITH
VERIFIED Q&A
PEA poor growth/no growth - ANSWER>>>organism inhibited by phenylethyl alc, probable gram -
organism
PEA good growth - ANSWER>>>organism is not inhibited by phenylethyl alc, probable staphylococcus,
streptococcus, enterococcus, or lactococcus
5% sheep blood poor growth - ANSWER>>>inhibited by colistin and nalidixic acid, probable gram -
5% sheep blood good growth - ANSWER>>>not inhibited by colistin and nalidixic acid and completely
hemolyzes RBCs, probable alpha hemolytic staph, strep, or entero
Mannitol Salt poor growth - ANSWER>>>organism inhibited by NaCl, not staph
mannitol salt good growth - ANSWER>>>organism not inhibited by NaCl, staph
Macconkey poor growth - ANSWER>>>organism is inhibited by crystal violet and or bile, gram +
macconkey good growth - ANSWER>>>organism is not inhibited by crystal violet or bile, gram +
EMB poor growth - ANSWER>>>organism is inhibited by eosin and methylene blue, gram +
EMB good growth - ANSWER>>>organism is not inhibited by eosin and methylene blue, gram -
Hektoen poor growth - ANSWER>>>organism is inhibited by bile and or one of the dyes included, gram +
hektoen good growth - ANSWER>>>organism isnt inhibited by bile or any of the dyes, gram -
Phenol red broth yellow with bubble - ANSWER>>>fermentation with acid and gas end products, A/G
Phenol Red broth yellow with no bubble - ANSWER>>>fermentation with acid end products, no gas
produced, A/-
phenol red broth red with no bubble - ANSWER>>>no fermentation, -/-
phenol red broth pink no bubble - ANSWER>>>degradation of peptone, alkaline end products, K
phenol red broth - ANSWER>>>will have glucose, lactose, or sucrose
methyl red broth red color - ANSWER>>>mixed acid fermentation, +
methyl red broth no color change - ANSWER>>>no mixed acid fermentation, -
APPLICATION TEST 2 WITH
VERIFIED Q&A
PEA poor growth/no growth - ANSWER>>>organism inhibited by phenylethyl alc, probable gram -
organism
PEA good growth - ANSWER>>>organism is not inhibited by phenylethyl alc, probable staphylococcus,
streptococcus, enterococcus, or lactococcus
5% sheep blood poor growth - ANSWER>>>inhibited by colistin and nalidixic acid, probable gram -
5% sheep blood good growth - ANSWER>>>not inhibited by colistin and nalidixic acid and completely
hemolyzes RBCs, probable alpha hemolytic staph, strep, or entero
Mannitol Salt poor growth - ANSWER>>>organism inhibited by NaCl, not staph
mannitol salt good growth - ANSWER>>>organism not inhibited by NaCl, staph
Macconkey poor growth - ANSWER>>>organism is inhibited by crystal violet and or bile, gram +
macconkey good growth - ANSWER>>>organism is not inhibited by crystal violet or bile, gram +
EMB poor growth - ANSWER>>>organism is inhibited by eosin and methylene blue, gram +
EMB good growth - ANSWER>>>organism is not inhibited by eosin and methylene blue, gram -
Hektoen poor growth - ANSWER>>>organism is inhibited by bile and or one of the dyes included, gram +
hektoen good growth - ANSWER>>>organism isnt inhibited by bile or any of the dyes, gram -
Phenol red broth yellow with bubble - ANSWER>>>fermentation with acid and gas end products, A/G
Phenol Red broth yellow with no bubble - ANSWER>>>fermentation with acid end products, no gas
produced, A/-
phenol red broth red with no bubble - ANSWER>>>no fermentation, -/-
phenol red broth pink no bubble - ANSWER>>>degradation of peptone, alkaline end products, K
phenol red broth - ANSWER>>>will have glucose, lactose, or sucrose
methyl red broth red color - ANSWER>>>mixed acid fermentation, +
methyl red broth no color change - ANSWER>>>no mixed acid fermentation, -
