©PREP4EXAMS@2024 [REAL-EXAM-DUMPS] Monday, July 29, 2024 12:15 PM
Microbiology Lab 1 Practical Study Guide
with Complete Solutions
Magnification - ✔️✔️makes an image appear larger then its actual size
Total magnification - ✔️✔️eyepiece x objective
wavelength of light - ✔️✔️Light travels as waves and wavelength is the distance peak to
peak on a wave
Visible light has a wavelength between 350-700 nanometers
Each wavelength is a different color of light
Violet = 350 nm, Red = 700nm
For good resolution, we use blue light (480/500 nm)
Numerical Aperture - ✔️✔️Ability of a lens to gather light
Working Distance - ✔️✔️space between the stage and the objective lens when the
specimen is in focus
Par focal - ✔️✔️once the specimen is in focus, you can change the objective lens and the
specimen remains in course focus
Field of View - ✔️✔️circle of light observed through microscope
As magnification increases, field of view decreases
Ocular Lens - ✔️✔️10x
Scanning Objective Lens - ✔️✔️4x
Lower power objective - ✔️✔️10x
1
, ©PREP4EXAMS@2024 [REAL-EXAM-DUMPS] Monday, July 29, 2024 12:15 PM
high power objective lens - ✔️✔️40x
oil immersion lens - ✔️✔️100x
Cocci - ✔️✔️spherical
Bacillus - ✔️✔️Rod Shape
spirilla - ✔️✔️spiral shape
Eubacteria - ✔️✔️true bacteria
Cyanbacteria - ✔️✔️make their own food by photosynthesis, they are bacteria but not
conform to any three shapes.
- consist of prokaryotic cell
Genus: Anabaena - ✔️✔️Name Genus and Phylum
Genus: Oscillatoria - ✔️✔️Name Genus and Phylum
Genus: Spirulina - ✔️✔️Name Genus and Phylum
Specie: Bacillus Subtilis - ✔️✔️Name genus, specie, and shape
Specie: Proteus vulgaris - ✔️✔️Name Species and genus
Specie: Staphylococcus aureus
Genus: Bacteria
Shape: cocci - ✔️✔️Name genus, specie, and shape
Streptococcus pyogenes
Genus: bacteria
Shape: cocci - ✔️✔️Name genus, specie, and shape
Species: Spirillum sp.
2
Microbiology Lab 1 Practical Study Guide
with Complete Solutions
Magnification - ✔️✔️makes an image appear larger then its actual size
Total magnification - ✔️✔️eyepiece x objective
wavelength of light - ✔️✔️Light travels as waves and wavelength is the distance peak to
peak on a wave
Visible light has a wavelength between 350-700 nanometers
Each wavelength is a different color of light
Violet = 350 nm, Red = 700nm
For good resolution, we use blue light (480/500 nm)
Numerical Aperture - ✔️✔️Ability of a lens to gather light
Working Distance - ✔️✔️space between the stage and the objective lens when the
specimen is in focus
Par focal - ✔️✔️once the specimen is in focus, you can change the objective lens and the
specimen remains in course focus
Field of View - ✔️✔️circle of light observed through microscope
As magnification increases, field of view decreases
Ocular Lens - ✔️✔️10x
Scanning Objective Lens - ✔️✔️4x
Lower power objective - ✔️✔️10x
1
, ©PREP4EXAMS@2024 [REAL-EXAM-DUMPS] Monday, July 29, 2024 12:15 PM
high power objective lens - ✔️✔️40x
oil immersion lens - ✔️✔️100x
Cocci - ✔️✔️spherical
Bacillus - ✔️✔️Rod Shape
spirilla - ✔️✔️spiral shape
Eubacteria - ✔️✔️true bacteria
Cyanbacteria - ✔️✔️make their own food by photosynthesis, they are bacteria but not
conform to any three shapes.
- consist of prokaryotic cell
Genus: Anabaena - ✔️✔️Name Genus and Phylum
Genus: Oscillatoria - ✔️✔️Name Genus and Phylum
Genus: Spirulina - ✔️✔️Name Genus and Phylum
Specie: Bacillus Subtilis - ✔️✔️Name genus, specie, and shape
Specie: Proteus vulgaris - ✔️✔️Name Species and genus
Specie: Staphylococcus aureus
Genus: Bacteria
Shape: cocci - ✔️✔️Name genus, specie, and shape
Streptococcus pyogenes
Genus: bacteria
Shape: cocci - ✔️✔️Name genus, specie, and shape
Species: Spirillum sp.
2
