(MB) ASCP PRACTICE EXAM
QUESTIONS AND ANSWERS WITH
SOLUTIONS 2024
Which of the following is not a component of a nucleotide?
Phosphate group
Anti-codon
Ribose sugar
Nitrogen base - ANSWER Anti-codon
According to Chargaff's rule of base pairing, adenine pairs with: - ANSWER Thymine
What genes would be screened in a breast cancer panel? - ANSWER HER2, ERBB2, BRCA1
Next Generation Sequencing uses: - ANSWER Short sequence reads
Purines and pyrimidines differ from each other in that: - ANSWER Purines have two rings; pyrimidines
have one ring
The purines are:
Cytosine and uracil
Adenine and thymine
Thymine and cytosine
Adenine and guanine - ANSWER Adenine and guanine
What is the rate of mutation per round of DNA replication?
1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
,1 in 1,000,000,000 base pairs - ANSWER 1 in 1,000,000,000 base pairs
The rate of DNA migration through an agarose gel during electrophoresis does not depend on which of
the following factors?
Net charge of the molecule
Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule - ANSWER Nucleotide sequence of the molecule
What are the phases in a qPCR Amplification Plot?
Initiation, exponential, plateau
Baseline, exponential, plateau
Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau - ANSWER Initiation, exponential, plateau
Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC - ANSWER 3'-GAC
A patient with impaired judgment, personality changes, signs of abnormal body movements and
depression comes to the physician's office for a follow-up visit. The physician suspects a single-gene
disorder may be the cause of those clinical manifestations. A blood specimen was then sent to your
clinical laboratory for mutation screening in the Huntington gene. Testing with standard PCR indicates
that patient has Huntington Disease, HD. Which of the following would be consistent with this diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
25 CGA repeats in the Huntington gene
,85 CGA repeats in the Huntington gene - ANSWER 85 CAG repeats in the Huntington gene
Which two HPV types are responsible for most cases of cervical cancer?
16 and 18
31 and 59
16 and 58
44 and 59 - ANSWER 16 and 18
Replication forks, known as origins of DNA replication, are created by this enzyme:
Ligase
Taq Polymerase
Primase
Helicase - ANSWER Helicase
Mutation in what gene is associated with Fragile X syndrome? - ANSWER FMR1
Mantle cell lymphoma (MCL) is caused by what translocation? - ANSWER t(11;14)
This polymerase is involved in "initiation of DNA replication and has primase activity": - ANSWER Pol α
Its discovery shed light on why there is simultaneous, though not continuous, synthesis of DNA on both
leading and lagging strands of DNA:
Klenow fragment of DNA polymerase
Okazaki fragments
Sanger fragments
RNA fragments - ANSWER Okazaki fragments
What gene is measured following treatment with imatinib (Gleevec)?
FLT3
, BCR/Abl
Jak2
MAPK - ANSWER BCR/Abl
What is the rate of mammalian DNA replication?
500 nucleotides per second
100 nucleotides per second
50 nucleotides per second
10 nucleotides per second - ANSWER 50 nucleotides per second
This polymerase is involved in "replicates mitochondrial DNA": - ANSWER Pol γ
A patient with impaired judgment, personality changes, signs of abnormal body movements and
depression comes to the physician's office for a follow-up visit. The physician suspects a single-gene
disorder may be the cause of those clinical manifestations. A blood specimen was then sent to your
clinical laboratory for mutation screening in the Huntingtin gene. Which of these methods would best
accomplish this task?
Methylation-specific PCR
Standard PCR
PFGE
RAPD PCR - ANSWER Standard PCR
Which of the following storage options is optimal for storing isolated DNA for a period greater than
seven years?
22-25ºC
2-8ºC
-20ºC
-70ºC - ANSWER -70ºC
Consider a hypothetical mutation involving gene X. Let's say you amplify a specific exon, say exon 11, of
that gene then you cut it with restriction enzyme W. In a person without the mutation, cutting the gene
QUESTIONS AND ANSWERS WITH
SOLUTIONS 2024
Which of the following is not a component of a nucleotide?
Phosphate group
Anti-codon
Ribose sugar
Nitrogen base - ANSWER Anti-codon
According to Chargaff's rule of base pairing, adenine pairs with: - ANSWER Thymine
What genes would be screened in a breast cancer panel? - ANSWER HER2, ERBB2, BRCA1
Next Generation Sequencing uses: - ANSWER Short sequence reads
Purines and pyrimidines differ from each other in that: - ANSWER Purines have two rings; pyrimidines
have one ring
The purines are:
Cytosine and uracil
Adenine and thymine
Thymine and cytosine
Adenine and guanine - ANSWER Adenine and guanine
What is the rate of mutation per round of DNA replication?
1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
,1 in 1,000,000,000 base pairs - ANSWER 1 in 1,000,000,000 base pairs
The rate of DNA migration through an agarose gel during electrophoresis does not depend on which of
the following factors?
Net charge of the molecule
Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule - ANSWER Nucleotide sequence of the molecule
What are the phases in a qPCR Amplification Plot?
Initiation, exponential, plateau
Baseline, exponential, plateau
Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau - ANSWER Initiation, exponential, plateau
Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC - ANSWER 3'-GAC
A patient with impaired judgment, personality changes, signs of abnormal body movements and
depression comes to the physician's office for a follow-up visit. The physician suspects a single-gene
disorder may be the cause of those clinical manifestations. A blood specimen was then sent to your
clinical laboratory for mutation screening in the Huntington gene. Testing with standard PCR indicates
that patient has Huntington Disease, HD. Which of the following would be consistent with this diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
25 CGA repeats in the Huntington gene
,85 CGA repeats in the Huntington gene - ANSWER 85 CAG repeats in the Huntington gene
Which two HPV types are responsible for most cases of cervical cancer?
16 and 18
31 and 59
16 and 58
44 and 59 - ANSWER 16 and 18
Replication forks, known as origins of DNA replication, are created by this enzyme:
Ligase
Taq Polymerase
Primase
Helicase - ANSWER Helicase
Mutation in what gene is associated with Fragile X syndrome? - ANSWER FMR1
Mantle cell lymphoma (MCL) is caused by what translocation? - ANSWER t(11;14)
This polymerase is involved in "initiation of DNA replication and has primase activity": - ANSWER Pol α
Its discovery shed light on why there is simultaneous, though not continuous, synthesis of DNA on both
leading and lagging strands of DNA:
Klenow fragment of DNA polymerase
Okazaki fragments
Sanger fragments
RNA fragments - ANSWER Okazaki fragments
What gene is measured following treatment with imatinib (Gleevec)?
FLT3
, BCR/Abl
Jak2
MAPK - ANSWER BCR/Abl
What is the rate of mammalian DNA replication?
500 nucleotides per second
100 nucleotides per second
50 nucleotides per second
10 nucleotides per second - ANSWER 50 nucleotides per second
This polymerase is involved in "replicates mitochondrial DNA": - ANSWER Pol γ
A patient with impaired judgment, personality changes, signs of abnormal body movements and
depression comes to the physician's office for a follow-up visit. The physician suspects a single-gene
disorder may be the cause of those clinical manifestations. A blood specimen was then sent to your
clinical laboratory for mutation screening in the Huntingtin gene. Which of these methods would best
accomplish this task?
Methylation-specific PCR
Standard PCR
PFGE
RAPD PCR - ANSWER Standard PCR
Which of the following storage options is optimal for storing isolated DNA for a period greater than
seven years?
22-25ºC
2-8ºC
-20ºC
-70ºC - ANSWER -70ºC
Consider a hypothetical mutation involving gene X. Let's say you amplify a specific exon, say exon 11, of
that gene then you cut it with restriction enzyme W. In a person without the mutation, cutting the gene
