ASCP EXAM STAIN QUESTIONS AND
ANSWERS WITH SOLUTIONS 2024
In colloidal iron method, principle is that formation of an ionic bond between ferric iron and the free
carboxyl group of what? - ANSWER Of acid mucopolysaccharides.
What is present in Congo red in order to suppress background staining and non specific staining? -
ANSWER Sodium chloride.
What happens if there is prolonged treatment in oxalic acid in a bodian technique? - ANSWER
Interference with primary staining can occur.
The reducing agent in diamine silver prosecuted for reticulum demonstration is usually what? - ANSWER
Formaldehyde.
What acid is used in an alcoholic solution to differentiate aluminum hematoxylin stained sections? -
ANSWER Hcl
What is a metachromatic stain used for identifying neurons and neuroglial cells? - ANSWER Toluidine
blue
A dye in which the coloring elements are found in the anionic portion of the compound is what kind of
dye? - ANSWER An acid dye.
True or false, mayer's hematoxylin is only used progressively? - ANSWER True
In a PTAH stain, skeletal muscle striations stain what color? - ANSWER Blue.
In a gram stain, when is iodine applied? - ANSWER It is applied after crystal violet.
True or false: toluidine blue is an example of a metachromatic stain? - ANSWER True
ANSWERS WITH SOLUTIONS 2024
In colloidal iron method, principle is that formation of an ionic bond between ferric iron and the free
carboxyl group of what? - ANSWER Of acid mucopolysaccharides.
What is present in Congo red in order to suppress background staining and non specific staining? -
ANSWER Sodium chloride.
What happens if there is prolonged treatment in oxalic acid in a bodian technique? - ANSWER
Interference with primary staining can occur.
The reducing agent in diamine silver prosecuted for reticulum demonstration is usually what? - ANSWER
Formaldehyde.
What acid is used in an alcoholic solution to differentiate aluminum hematoxylin stained sections? -
ANSWER Hcl
What is a metachromatic stain used for identifying neurons and neuroglial cells? - ANSWER Toluidine
blue
A dye in which the coloring elements are found in the anionic portion of the compound is what kind of
dye? - ANSWER An acid dye.
True or false, mayer's hematoxylin is only used progressively? - ANSWER True
In a PTAH stain, skeletal muscle striations stain what color? - ANSWER Blue.
In a gram stain, when is iodine applied? - ANSWER It is applied after crystal violet.
True or false: toluidine blue is an example of a metachromatic stain? - ANSWER True
