Microbiology Lab UTA - Final Exam; Questions
and Answers % Correct
How is Media made? Correct answer-5 g. T.S.A. and 125 mL Di water, cover flask with foil,
affix autoclave tape, autoclave, wait 10-20 min to cool, pour into petri-dish to thickness of lid. or
stop when covers bottom.
Agar Correct answer-linear polysaccharide polymer derived from agarose in red algae.
What does agar do? Correct answer-increases solidity, exhibits hysteresis- gels and melts
at different temps. Melts above 85-90 and solidifies at 40-45.
What is liquid media? Correct answer-commonly called broths, doesn't contain agar, easy
to use, allows observation of growth patterns.
Advantages to using semi-solid media Correct answer-To test oxygen requirements, to
test motility.
Fastidious Correct answer-only grows in a fortified culture media under specific
conditions. The more _____________ the organism, the more specific ingredients a medium
must have. Uses defined media.
Why use solid media? Correct answer-The maintenance and growth of stock cultures, to
isolate single colonies, to ensure purity.
, Microbiology Lab UTA - Final Exam; Questions
and Answers % Correct
T.S.A. Plate Correct answer-Tryptic Soy Agar most common medium used in lab.
Undefined media that contains casein digest, soybean digest, NaCl, and agar.
What are the 4 biochemical families all living things are composed of? Correct answer-
Proteins, Carbohydrates, lipids, nucleic acids
How much water does a bacteria cell consist of? Correct answer-70%
Autoclave Correct answer-sterilizes with steam at 121* C, pressure at 15 PSI for at least
15 min. Tested weekly to daily.
Reservoir Correct answer-potential source of infection.
Normal commensal symbionts Correct answer-S. pneumoniae and s. aureus
Environmentally acquired microbes Correct answer-p. aeruginosa and b. cepacia
Magnifications of our microscopes Correct answer-scanning: 4x-40, low: 10x-100, high:
40x-400, oil immersion: 100x-1000
What is resolving power? Correct answer-How close two objects can be and still be
distinguished as separate.
, Microbiology Lab UTA - Final Exam; Questions
and Answers % Correct
What is the optimum resolution of the microscope using oil immersion? Correct answer-
.2 micrometer.
How much does each microscope cost? Correct answer-$1600
convex Correct answer-Morphology of a domed colony
raised Correct answer-flatter dome than convex
flat elevation Correct answer-
crateriform Correct answer-hole in convex colony
Umbonate Correct answer-Morphology of a colony with a raised center
Rhizoid Correct answer-Morphology of a branching colony
lobate Correct answer-margin with lobes
, Microbiology Lab UTA - Final Exam; Questions
and Answers % Correct
undilated margins Correct answer-irregular but not lobed
curled Correct answer-wave pattern on margin
entire margin Correct answer-even smooth all around
Filiform Growth Correct answer-Agar slant. Colony exhibits a dense/opaque surface with
a defined edge.
Friable Growth Correct answer-Agar slants. Colony exhibits crusty, irregular edges.
Spreading edges Correct answer-motile organism
Human error in inoculation Correct answer-What is responsible for most irregularities in
agar slants?
Five I's Correct answer-inoculation, incubation, isolation, inspection, identification
Flocculence Correct answer-Clumpy colonial aggregates seen in broth media.
streptococcus
and Answers % Correct
How is Media made? Correct answer-5 g. T.S.A. and 125 mL Di water, cover flask with foil,
affix autoclave tape, autoclave, wait 10-20 min to cool, pour into petri-dish to thickness of lid. or
stop when covers bottom.
Agar Correct answer-linear polysaccharide polymer derived from agarose in red algae.
What does agar do? Correct answer-increases solidity, exhibits hysteresis- gels and melts
at different temps. Melts above 85-90 and solidifies at 40-45.
What is liquid media? Correct answer-commonly called broths, doesn't contain agar, easy
to use, allows observation of growth patterns.
Advantages to using semi-solid media Correct answer-To test oxygen requirements, to
test motility.
Fastidious Correct answer-only grows in a fortified culture media under specific
conditions. The more _____________ the organism, the more specific ingredients a medium
must have. Uses defined media.
Why use solid media? Correct answer-The maintenance and growth of stock cultures, to
isolate single colonies, to ensure purity.
, Microbiology Lab UTA - Final Exam; Questions
and Answers % Correct
T.S.A. Plate Correct answer-Tryptic Soy Agar most common medium used in lab.
Undefined media that contains casein digest, soybean digest, NaCl, and agar.
What are the 4 biochemical families all living things are composed of? Correct answer-
Proteins, Carbohydrates, lipids, nucleic acids
How much water does a bacteria cell consist of? Correct answer-70%
Autoclave Correct answer-sterilizes with steam at 121* C, pressure at 15 PSI for at least
15 min. Tested weekly to daily.
Reservoir Correct answer-potential source of infection.
Normal commensal symbionts Correct answer-S. pneumoniae and s. aureus
Environmentally acquired microbes Correct answer-p. aeruginosa and b. cepacia
Magnifications of our microscopes Correct answer-scanning: 4x-40, low: 10x-100, high:
40x-400, oil immersion: 100x-1000
What is resolving power? Correct answer-How close two objects can be and still be
distinguished as separate.
, Microbiology Lab UTA - Final Exam; Questions
and Answers % Correct
What is the optimum resolution of the microscope using oil immersion? Correct answer-
.2 micrometer.
How much does each microscope cost? Correct answer-$1600
convex Correct answer-Morphology of a domed colony
raised Correct answer-flatter dome than convex
flat elevation Correct answer-
crateriform Correct answer-hole in convex colony
Umbonate Correct answer-Morphology of a colony with a raised center
Rhizoid Correct answer-Morphology of a branching colony
lobate Correct answer-margin with lobes
, Microbiology Lab UTA - Final Exam; Questions
and Answers % Correct
undilated margins Correct answer-irregular but not lobed
curled Correct answer-wave pattern on margin
entire margin Correct answer-even smooth all around
Filiform Growth Correct answer-Agar slant. Colony exhibits a dense/opaque surface with
a defined edge.
Friable Growth Correct answer-Agar slants. Colony exhibits crusty, irregular edges.
Spreading edges Correct answer-motile organism
Human error in inoculation Correct answer-What is responsible for most irregularities in
agar slants?
Five I's Correct answer-inoculation, incubation, isolation, inspection, identification
Flocculence Correct answer-Clumpy colonial aggregates seen in broth media.
streptococcus
