PAG 9.1 – Protein Questions and Answers | Latest Version | Graded A+
PAG 9.1 – Protein Questions and Answers | Latest Version | Graded A+ **What are the components of Biuret reagent, and which chemicals pose hazards?** Biuret reagent contains sodium hydroxide, which is highly alkaline and can be corrosive in concentrated form. Copper sulfate is also present, which can be toxic to animals and harmful to humans. **Why is it necessary to observe the Biuret reagent as a layer on top of the sample before mixing?** This step allows for easier identification of proteins by observing any color change. If the sample already appears blue, it can obscure the detection of protein presence. **What is the biochemical explanation behind a positive Biuret test result?** The positive result occurs because copper(II) ions in the Biuret reagent react with peptide bonds' nitrogen atoms, forming a colored complex. **What color indicates the presence of proteins in the Biuret test?** Purple
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