Bio 250 Midterm Questions And Answers With Latest Study Solutions

mmunochemistry - ANS-used to find antigens, proteins, and cancer in specific tissues via antibodies induced pluripotent stem-cell programming - ANS-tweaks adult cells into stem cells to force them to express genes, to have properties of embryonic stem cells, to model disease and study drug development RT-qPCR - ANS-used to analyze gene expression (how much is gene expressed?) real time PCR - ANS-measures telomere length in humans western blot - ANS-detects presence of specific proteins in a tissue sample transfection - ANS-gene control/silencing -virus production -protein production -differentiation viral vector - ANS-insertion of genes -over expression of genes Cre-lox - ANS-deletions, insertions, translations and inversions of specific sites of DNA how is cre-lox different from viral vectors, transfection or RNAi? - ANS-broader manipulation of genome sandwich ELISA - ANS-tests presence of antigens or antibodies -measure concentration of molecules and proteins RNAi - ANS-used to knockout genes at a translational level northern blot - ANS-studies gene expression via detection of RNA -determines the presence of an expressed gene in a sample