CHEM 121 Lab 3 Quantitative and Qualitative Analysis (Portage learning) SPRING

Experiment # 3 Quantitative and Qualitative Analysis Purpose: Using tools to measure and identify substances. Procedure: A. Measuring quantity of a substance to identify metal carbonate. 1) Tare test tube in a beaker and measure out approximately 1.1809 gram of unknown metal carbonate into test tube. 2) Add sulfuric acid to the beaker and mix. Watch reaction for CO2 gas evaporation. 3) Measure weight of mixture and record the difference. 4) Use formula to calculate %CO2 in the sample=weight loss/original weight x 100 5) Choose the most likely compound used in the mystery substance based on percentage of CO2 composition. CaCO3, KCO3, Na2CO3, Li2CO3 B. Separation by paper chromatography. 1) Measure weight of crucible, tare, and add 2.4406 g of a metal hydrate 2) Place crucible on ring stand with burner underneath. Loosely place the cover of the crucible on top and let heat for 10 minutes. 3) Using tongs, place the crucible in the desiccator and allow to cool for 10 minutes. 4) Place the crucible in the analytical scale and measure the new weight. 5) Use the formula %H2O= difference in weight/original weight x 100 6) Using the %H2O composition, identify the most likely metal hydrate used in the substance: AlCl3, MgCl2, Na2CO3, BaCl2 C. Qualitative analysis to identify substance using paper chromatography. 1) Don gloves. Use porous paper (stationary phase) that has had samples of amino acids applied to it along the bottom of the paper and staple in a roll. 2) Mark the bottom line of the roll. 3) Place porous paper roll in a beaker filled with a solution (mobile phase) of butanol, acetic acid and