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Samenvatting Moleculaire Biologie Technieken

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Written in
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Samenvatting Moleculaire Biologie Technieken. Met deze samenvatting heb ik een 7,6 gehaald. De leerdoelen zijn verwerkt in het document. TLSC-MOLBIO3V-16

Institution
Hogeschool Utrecht (HU)
Course
Moleculaire biologie technieken (TLSCMOLBIO3V16)

Content preview

Inhoud
Les 1, Algemene DNA Technieken..........................................................................................................2
Les 2, Algemene DNA Technieken..........................................................................................................4
Les 3, PCR...............................................................................................................................................8
Les 4, Sequencen..................................................................................................................................11
Les 5, Opsporen van Genen en Functiebepaling...................................................................................12
Les 6, CRISPR/Cas.................................................................................................................................14




1

, Les 1, Algemene DNA
Technieken
Kloneren:

Het produceren van genetische identieke
organismen.

Moleculair kloneren:
Het genereren van recombinant DNA en de
replicatie daarvan in gast organismen,
gebruikmakend van moleculair biologische
technieken.
Amplificatie door bacteriën:

Plasmide DNA als kloneervector:

Nieuwe genen genereren, vermenigvuldigen
en tot expressie brengen.

Bevat:

- ORI: bindingsplaats voor DNApol,
startplaats replicatie.
- MCS: unieke knipsites.
- Selectiemarkers.
Recombinant DNA technologie:

Enzymen:

- Nucleases (restrictie-enzymen),
knippen
- Modificatoren, bewerken
- Ligases, plakken
- Polymerases, kopiëren

Nucleases:

- Endo- en exonucleases
- Knipt tussen desoxyribose en
fosfaatgroep.
- Bacterie beschermt zichzelf door A en
C te methyleren.

Restrictie-enzymen:


2
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Connected book

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Bruce Alberts, Alexander Johnson Molecular Biology of the Cell
  • december 2014
  • 9780815344643
  • 6

Written for

Institution
Hogeschool Utrecht (HU)
Study
Life Science & Technology
Course
Moleculaire biologie technieken (TLSCMOLBIO3V16)

Document information

Summarized whole book?
No
Which chapters are summarized?
Bijbehorende hoofdstukken
Uploaded on
June 9, 2019
Number of pages
14
Written in
2018/2019
Type
SUMMARY

Subjects

  • tlsc molbio3v 16
  • molbio
  • molbio life sciences
  • moleculaire biologie
  • moleculaire biologie technieken
  • moleculaire biologie technieken life sciences
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Bundel Moleculaire Biologie Theorie + Technieken, verwerkte leerdoelen
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2 2 2019
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Hoi! Ik ben een derdejaars student aan Hogeschool Utrecht en studeer Life Sciences, oftewel Biomedisch Laboratoriumonderzoek. Momenteel doe ik de specialisatie microbiologie. Met mijn samenvattingen heb ik alleen maar voldoendes gehaald en zo wil ik ook andere studenten motiveren. Mijn samenvattingen maak ik aan de hand van de colleges die ik volg en het boek dat bij het vak hoort.

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