BIOMG 3300 - Unit 9 Questions with complete Solutions

Describe the five general procedures in DNA cloning Correct Answer-1. Obtaining the DNA segment to be cloned 2. Selecting a small molecule of DNA capable of autonomous replication 3. Joining two DNA fragments covalently 4. Moving recombinant DNA from the test tube to a host organism 5. Selecting or identifying host cells that contain recombinant DNA Are restriction enzymes made by eukaryotes or prokaryotes? What is their function in vivo? Correct Answer-Restriction endonucleases are found in a wide range of bacterial species their biological function is to recognize and cleave foreign DNA - In the host cell's DNA, the sequence that would be recognized by one of its own restriction endonucleases is protected from digestion by methylation of the DNA, catalyzed by a specific DNA methylase Some restriction endonucleases make staggered cuts on the two DNA strands, leaving two to four nucleotides of one strand unpaired at each resulting end because they can base-pair with each other or with complementary sticky ends of other DNA fragments - sticky ends