Task 2, 1
11053457
Task 2 Carry out and write up the following experiment:
1, Chromatography to separate sugars
What chromatography to separate sugars is:
Employing filter-paper chromatography a convenient method (Horizontal Migration Method)
has been developed for the identification of reducing sugars, when present either alone or in
mixtures.
Aim:
The purpose of applying chromatography which is used as a method of quantitative analysis
apart from its separation, is to achieve a satisfactory separation within a suitable time
interval. Various chromatographic methods have been developed to that end. Some of them
include column chromatography, thin-layer chromatography (TLC), paper chromatography,
gas chromatography, ion exchange chromatography, gel permeation chromatography, high-
pressure liquid chromatography, and affinity chromatography
Materials:
● Paper : Usually whatman No. 1 filter paper is used because of its known
● Solvents: [a] Water-saturated phenol + 1% ammonia [b] n-butanol-acetic acid-water
(4:1:5 v/v) [c] isopropanol-pyridine-water-acetic acid (8:8:4:1 v/v)
● Spray reagent: Ninhydrin
● Beaker: 250ml
● Pencil,Ruler
● TLC plate
● Clingfilm
● Oven
● Hair dryer
● Fume cupboard
● Capillary tubes
● 3 different sugars, Glucose, Galactose and Maltose, and a Mixture.
11053457
Task 2 Carry out and write up the following experiment:
1, Chromatography to separate sugars
What chromatography to separate sugars is:
Employing filter-paper chromatography a convenient method (Horizontal Migration Method)
has been developed for the identification of reducing sugars, when present either alone or in
mixtures.
Aim:
The purpose of applying chromatography which is used as a method of quantitative analysis
apart from its separation, is to achieve a satisfactory separation within a suitable time
interval. Various chromatographic methods have been developed to that end. Some of them
include column chromatography, thin-layer chromatography (TLC), paper chromatography,
gas chromatography, ion exchange chromatography, gel permeation chromatography, high-
pressure liquid chromatography, and affinity chromatography
Materials:
● Paper : Usually whatman No. 1 filter paper is used because of its known
● Solvents: [a] Water-saturated phenol + 1% ammonia [b] n-butanol-acetic acid-water
(4:1:5 v/v) [c] isopropanol-pyridine-water-acetic acid (8:8:4:1 v/v)
● Spray reagent: Ninhydrin
● Beaker: 250ml
● Pencil,Ruler
● TLC plate
● Clingfilm
● Oven
● Hair dryer
● Fume cupboard
● Capillary tubes
● 3 different sugars, Glucose, Galactose and Maltose, and a Mixture.
