Unit 13: Task 3
Iman Boukjija Zamani
11053457
Results:
Substances Distance moved by each spot (cm)
Glucose 2.5
Galactose 2.2
Maltose 2
Mixture (2 different substances) 2, 2.5
Calculations: The compounds can be identified using their retention factors, Rf . These
values can be worked out by measuring the distance the solvent has moved up the sheet
and the distance each spot has moved up the sheet. This is shown in the following equation:
Rf=Distance travelled by compound
Distance travelled by solvent
Distance travelled by solvent=5cm
Glucose Galactose Maltose Unknown substance 1
Rf=2.5/5 Rf=2.2/5 Rf=2/5 Rf=2/5
=0.5 = 0.44 = 0.4 =0.4
Unknown substance 2
Rf=2.5/5
=0.5
Risk assessment:
Hazard Risk Control Measures Actions if it goes wrong
The solvent (made It is highly flammable Gloves must be worn. Wash your hands
up of Ethyl Acetate, immediately.
Isopropanol, Pyridine
and Water).
Ninhydrin Spray It is highly flammable Wear suitable If the spray has reached
Causes serious eye irritation protective clothing. your eyes immediately
May cause Avoid breathing rinse with water for several
drowsiness/dizziness vapours, mist or gas. minutes.
Avoid formation of dust.
Ensure adequate
ventilation of the
working area. Evacuate
personnel to a safe
Iman Boukjija Zamani
11053457
Results:
Substances Distance moved by each spot (cm)
Glucose 2.5
Galactose 2.2
Maltose 2
Mixture (2 different substances) 2, 2.5
Calculations: The compounds can be identified using their retention factors, Rf . These
values can be worked out by measuring the distance the solvent has moved up the sheet
and the distance each spot has moved up the sheet. This is shown in the following equation:
Rf=Distance travelled by compound
Distance travelled by solvent
Distance travelled by solvent=5cm
Glucose Galactose Maltose Unknown substance 1
Rf=2.5/5 Rf=2.2/5 Rf=2/5 Rf=2/5
=0.5 = 0.44 = 0.4 =0.4
Unknown substance 2
Rf=2.5/5
=0.5
Risk assessment:
Hazard Risk Control Measures Actions if it goes wrong
The solvent (made It is highly flammable Gloves must be worn. Wash your hands
up of Ethyl Acetate, immediately.
Isopropanol, Pyridine
and Water).
Ninhydrin Spray It is highly flammable Wear suitable If the spray has reached
Causes serious eye irritation protective clothing. your eyes immediately
May cause Avoid breathing rinse with water for several
drowsiness/dizziness vapours, mist or gas. minutes.
Avoid formation of dust.
Ensure adequate
ventilation of the
working area. Evacuate
personnel to a safe
