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Portage Learning BIO 171- Microbiology Lab Notebook Lab Notebook Bookmarks (click to navigate

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2023/2024 Written for academic year
Institution Portage Learning BIO 171- Miteboo
Course Portage Learning BIO 171- Miteboo
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Lab 1 Notebook Back to Home Page Title: KD01 Introduction to medical microbiology & lab notebook procedures Course Objectives: Cultivation of samples: growth conditions and equipment used Identification of samples: biochemical assays & tests available Evaluation of samples: microscopy- visualization and key characteristics ofspecimens Basic Equipment: Cleaning: Autoclave 125°C- uses heat, pressure, & steam to sterilize; autoclave will take minutes to sterilize & hot dry air which will take hours; before opening make sure it is depressurized and will have very hot air. Growing (bacteria/pathogens) – Fixed incubator 37°C is airtight, shelving for petri dishes - Shaker incubator 37°C shakes culture & rotatesto aerateliquid nutrient broth Visualizing: microscopy Storing: refrigerator @ 4°C to stop the growth and helps preserve samples & keeps long term Lab Safety 1. Never eat or drink in the lab: contamination risks 2. Use PPE (personal protection equipment): gloves- latex, nitrile (purple, blue, green),thermal, or cold gloves (liquid nitrogen): eyewear: lab coat- chemical spills 2 lOMoAR cPSD| 3. Never leave lab while wearing PPE: including bathroom, hallway, or cafeteria

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