DNA Structure and Organization-Questions and Answers 100% Verified
DNA Structure and Organization-Questions and Answers 100% Verified For a molecule to serve as the genetic material it must exhibit what 4 key characteristics? -ANSWER-1. replicate 2. store information 3. express information 4. allow variation by mutation What processes make up the central dogma of molecular genetics? -ANSWER-Transcription & Translation What was previously thought to be the genetic material? Why? -ANSWER-protein; DNA was thought to be too simple with only 4 types of nucleotide vs 20 different amino acids in proteins how was evidence favoring DNA as genetic material obtained? -ANSWER-Griffith (1927) showed that avirulent strains of Diplococcus pneumoniae could be transformed to virulence. He speculated that the transforming principle could be part of the polysaccharide capsule or some compound required for capsule synthesis What did Hershey and Chase demonstrate? How? -ANSWER-DNA alone directs bacteriophage virus reproduction in the infection of E. coli. By differentially radiolabelling DNA and protein, transfection of viral DNA into bacterial cells proved conclusively that the viral DNA alone contains all the necessary information for production of mature viruses The strongest direct evidence for DNA as the genetic material comes from.. -ANSWER-recombinant DNA technology what is recombinant DNA technology? Give an example -ANSWER--eukaryotic DNA corresponding to specific genes are isolated and spliced into the bacterial DNA and can be inserted into a bacterial cell and monitored -presence of the eukaryotic gene product in bacteria containing the eukaryotic gene provides direct evidence that this DNA is present and functional in the bacterial cell -has been shown to occur in many instances ie. insulin production by bacteria How do viruses with RNA rather than DNA core replicate? -ANSWER-via RNA replicase How do retroviruses replicate? -ANSWER-RNA serves as a template for synthesis of complementary DNA by the RNA-dependent DNA polymerase reverse transcriptase. This DNA can be incorporated into the host cell genome
Document information
- Uploaded on
- March 25, 2024
- Number of pages
- 6
- Written in
- 2023/2024
- Type
- Exam (elaborations)
- Contains
- Questions & answers
