CBSPD Sterilization - Certification Exam
Review
Steam Using Gravity Displacement: Method of Killing - ANSDenatures microorganism proteins
Steam Using Gravity Displacement: Sterilant Used - ANSCombination of steam and ambient air
Steam Using Gravity Displacement: How do steam and ambient are sterilize? - ANSSteam
displaces the air, and forces it out of the drain vent. Steam then comes in direct contact with
items
Steam Using Gravity Displacement: Parameters (3) - ANS1. Time
2. Temperature
3. Pressure
Steam Using Gravity Displacement: Common cycle times (3) - ANS1. 30 min, 250°F, 15 psi
2. 15-25 min, 275°F, 27 psi
3. 10 min, 275°F, 30 psi
Steam Using Gravity Displacement: Items that CAN be sterilized - ANS1. Critical items that are
heat and moisture resistant
2. Semi-critical items that are heat and moisture resistant
3. Sharps Containers
Steam Using Gravity Displacement: Items that CANNOT be sterilized - ANSHeat and moisture
sensitive materials
Steam Using Gravity Displacement: Biological Indicator - ANSGeobacillus Stearothermophilus
Steam Using Dynamic Air Removal: Method of Killing - ANSDenatures microorganism proteins
Steam Using Dynamic Air Removal: Sterilants Used (3) - ANS1. Combination of steam and
ambient air
2. Prevacuum Sterilizers
3. (SFPP) Steam-flush pressure-pulse sterilizers
Steam Using Dynamic Air Removal: Parameters (3) - ANS1. Time
2. Temperature
3. Pressure
, Steam Using Dynamic Air Removal: Common Cycle Times (2) - ANS1. 4 min, 270°F, 28-30 psi
2. 3 min, 275°F, 28-30 psi
Steam Using Dynamic Air Removal: Items that CAN be sterilized - ANS1. Critical items that are
heat and moisture resistant
2. Semi-critical items that are heat and moisture resistant
3. Sharps Containers
Steam Using Dynamic Air Removal: Items that CANNOT be sterilized - ANSHeat and moisture
sensitive items
Steam Using Dynamic Air Removal: Biological Indicator - ANSGeobacillus Stearothermophilus
EO: Method of Killing - ANSAlkylation: interferes with microorganism metabolism
EO: Sterilants Used (2) - ANS1. 100% EO (undiluted) - common
2. EO Mixtures (contains HCFC and CO2) - uncommon
EO: Parameters (5) - ANS1. Time
2. Temperature
3. Pressure
4. Humidity
5. Concentration
EO: Common cycle time - ANS2.5 hours (not including aeration time)
EO: Items that CAN be sterilized (3) - ANS1. Critical items that are heat, pressure, and moisture
sensitive
2. Semi-critical items that are heat, pressure, and moisture sensitive
3. Devices with long lumens
EO: Items that CANNOT be sterilized - ANSTrays with silicone mats (must follow manufacturer's
guidelines)
EO: Biological Indicator - ANSBacillus Atrophaeus
Gas Plasma: Method of Killing - ANSInterferes with microorganism metabolism by
compromising essential components (enzymes, nucleic acid, etc.)
Gas Plasma: Sterilant Used - ANSCombination of hydrogen peroxide vapor and gas plasma
Gas Plasma: Parameters (3) - ANS1. Time
2. Temperature
3. Hydrogen Peroxide Gas Plasma
Review
Steam Using Gravity Displacement: Method of Killing - ANSDenatures microorganism proteins
Steam Using Gravity Displacement: Sterilant Used - ANSCombination of steam and ambient air
Steam Using Gravity Displacement: How do steam and ambient are sterilize? - ANSSteam
displaces the air, and forces it out of the drain vent. Steam then comes in direct contact with
items
Steam Using Gravity Displacement: Parameters (3) - ANS1. Time
2. Temperature
3. Pressure
Steam Using Gravity Displacement: Common cycle times (3) - ANS1. 30 min, 250°F, 15 psi
2. 15-25 min, 275°F, 27 psi
3. 10 min, 275°F, 30 psi
Steam Using Gravity Displacement: Items that CAN be sterilized - ANS1. Critical items that are
heat and moisture resistant
2. Semi-critical items that are heat and moisture resistant
3. Sharps Containers
Steam Using Gravity Displacement: Items that CANNOT be sterilized - ANSHeat and moisture
sensitive materials
Steam Using Gravity Displacement: Biological Indicator - ANSGeobacillus Stearothermophilus
Steam Using Dynamic Air Removal: Method of Killing - ANSDenatures microorganism proteins
Steam Using Dynamic Air Removal: Sterilants Used (3) - ANS1. Combination of steam and
ambient air
2. Prevacuum Sterilizers
3. (SFPP) Steam-flush pressure-pulse sterilizers
Steam Using Dynamic Air Removal: Parameters (3) - ANS1. Time
2. Temperature
3. Pressure
, Steam Using Dynamic Air Removal: Common Cycle Times (2) - ANS1. 4 min, 270°F, 28-30 psi
2. 3 min, 275°F, 28-30 psi
Steam Using Dynamic Air Removal: Items that CAN be sterilized - ANS1. Critical items that are
heat and moisture resistant
2. Semi-critical items that are heat and moisture resistant
3. Sharps Containers
Steam Using Dynamic Air Removal: Items that CANNOT be sterilized - ANSHeat and moisture
sensitive items
Steam Using Dynamic Air Removal: Biological Indicator - ANSGeobacillus Stearothermophilus
EO: Method of Killing - ANSAlkylation: interferes with microorganism metabolism
EO: Sterilants Used (2) - ANS1. 100% EO (undiluted) - common
2. EO Mixtures (contains HCFC and CO2) - uncommon
EO: Parameters (5) - ANS1. Time
2. Temperature
3. Pressure
4. Humidity
5. Concentration
EO: Common cycle time - ANS2.5 hours (not including aeration time)
EO: Items that CAN be sterilized (3) - ANS1. Critical items that are heat, pressure, and moisture
sensitive
2. Semi-critical items that are heat, pressure, and moisture sensitive
3. Devices with long lumens
EO: Items that CANNOT be sterilized - ANSTrays with silicone mats (must follow manufacturer's
guidelines)
EO: Biological Indicator - ANSBacillus Atrophaeus
Gas Plasma: Method of Killing - ANSInterferes with microorganism metabolism by
compromising essential components (enzymes, nucleic acid, etc.)
Gas Plasma: Sterilant Used - ANSCombination of hydrogen peroxide vapor and gas plasma
Gas Plasma: Parameters (3) - ANS1. Time
2. Temperature
3. Hydrogen Peroxide Gas Plasma
