MLPAO Test Prep ~ Histology and Cytology Accurate 100%(RATED A)
Histology Definition - ANSWER Study of the structure of tissues
Histology: Types of specimens received - ANSWER Surgical procedures, biopsies, autopsies
Histology: Surgical specimens include - ANSWER Whole or part of an organ, cysts, tumors
Histology: Biopsy specimens include - ANSWER Removal of a representative sample (through needle aspiration, surgery, or endoscopy)
Histology: Autopsy specimens include - ANSWER Removal of tissue from a deceased person (to analyze the cause of death, diagnosis, or for research)
Specimens must be labelled with - ANSWER First and last name, DOB, pt address, Unique ID number, OHIP number, Specimen type, Specimen number
Specimens are assigned a ____ number through LIS - ANSWER Surgical
Specimens must have a _______ with them - ANSWER requisition
Requisition / on-line request must contain the following - ANSWER pt first and last name, DOB, Unique ID Number, OHIP number, pt Address, pt location, ordering physician, specimen type, specimen number (if there is more than one specimen)
Specimens arrive in the lab as either a ___ or ____ specimen - ANSWER Fixed, unfixed
What is the first step when dealing with processing histology specimens? - ANSWER Verify patient information of requisition
Act in which components of cells and tissues are fixed in a physical and somewhat chemical state to endure treatment, avoiding loss, alteration, and decomposition - ANSWER Fixation
Specimens should be placed in a fixative that is ____ the estimated volume of the tissue
- ANSWER 10-20X
What is considered to be the universal fixative? - ANSWER Formalin/formaldehyde Fixation preserves tissue in a - ANSWER lifelike manner
Fixation prevents - ANSWER putrefaction and autolysis
Autolysis definition - ANSWER Decomposition due to the action of enzymes
Putrefaction definition - ANSWER Decomposition due to the action of bacteria
Fixation denatures or precipitates ___ - ANSWER Proteins
Fixation: Go through beneficial ___ - ANSWER hardening
Fixation: Enhances ____ procedures - ANSWER staining
Fixation: ____ microorganisms - ANSWER fixes
Advantages of other fixatives: B5 - ANSWER Displays good nuclear detail
Advantages of other fixatives: Glutaraldehyde - ANSWER Used in electron microscopy
Fixative that fixes tissues for cross-linking proteins - ANSWER Glutaraldehyde
Grossing of specimens Record the description of tissue including - ANSWER Size, color, consistency, and any abnormalities (if present)
Grossing of specimens tissues are - ANSWER Sliced open and viewed
Grossing of specimens biopsies and smaller sample handling - ANSWER placed in lens
paper
Decalcification definition - ANSWER Controlled removal of calcium from the tissue
Tissue must be ____ before being decalcified - ANSWER Fixed
Why must tissue be fixed before decalcification? - ANSWER Cell morphology will be damaged by the strong strength of the decalcification solution
Examples of decalcification agents - ANSWER Nitric acid, formic acid, EDTA
Dehydration definition - ANSWER Controlled removal of water from the tissue
Dehydration has tissue placed in increasing strengths of _____ to remove the water completely - ANSWER alcohol
Dehydration %s - ANSWER 70, 85, 95, 100
Histology Definition - ANSWER Study of the structure of tissues
Histology: Types of specimens received - ANSWER Surgical procedures, biopsies, autopsies
Histology: Surgical specimens include - ANSWER Whole or part of an organ, cysts, tumors
Histology: Biopsy specimens include - ANSWER Removal of a representative sample (through needle aspiration, surgery, or endoscopy)
Histology: Autopsy specimens include - ANSWER Removal of tissue from a deceased person (to analyze the cause of death, diagnosis, or for research)
Specimens must be labelled with - ANSWER First and last name, DOB, pt address, Unique ID number, OHIP number, Specimen type, Specimen number
Specimens are assigned a ____ number through LIS - ANSWER Surgical
Specimens must have a _______ with them - ANSWER requisition
Requisition / on-line request must contain the following - ANSWER pt first and last name, DOB, Unique ID Number, OHIP number, pt Address, pt location, ordering physician, specimen type, specimen number (if there is more than one specimen)
Specimens arrive in the lab as either a ___ or ____ specimen - ANSWER Fixed, unfixed
What is the first step when dealing with processing histology specimens? - ANSWER Verify patient information of requisition
Act in which components of cells and tissues are fixed in a physical and somewhat chemical state to endure treatment, avoiding loss, alteration, and decomposition - ANSWER Fixation
Specimens should be placed in a fixative that is ____ the estimated volume of the tissue
- ANSWER 10-20X
What is considered to be the universal fixative? - ANSWER Formalin/formaldehyde Fixation preserves tissue in a - ANSWER lifelike manner
Fixation prevents - ANSWER putrefaction and autolysis
Autolysis definition - ANSWER Decomposition due to the action of enzymes
Putrefaction definition - ANSWER Decomposition due to the action of bacteria
Fixation denatures or precipitates ___ - ANSWER Proteins
Fixation: Go through beneficial ___ - ANSWER hardening
Fixation: Enhances ____ procedures - ANSWER staining
Fixation: ____ microorganisms - ANSWER fixes
Advantages of other fixatives: B5 - ANSWER Displays good nuclear detail
Advantages of other fixatives: Glutaraldehyde - ANSWER Used in electron microscopy
Fixative that fixes tissues for cross-linking proteins - ANSWER Glutaraldehyde
Grossing of specimens Record the description of tissue including - ANSWER Size, color, consistency, and any abnormalities (if present)
Grossing of specimens tissues are - ANSWER Sliced open and viewed
Grossing of specimens biopsies and smaller sample handling - ANSWER placed in lens
paper
Decalcification definition - ANSWER Controlled removal of calcium from the tissue
Tissue must be ____ before being decalcified - ANSWER Fixed
Why must tissue be fixed before decalcification? - ANSWER Cell morphology will be damaged by the strong strength of the decalcification solution
Examples of decalcification agents - ANSWER Nitric acid, formic acid, EDTA
Dehydration definition - ANSWER Controlled removal of water from the tissue
Dehydration has tissue placed in increasing strengths of _____ to remove the water completely - ANSWER alcohol
Dehydration %s - ANSWER 70, 85, 95, 100
