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Samenvatting Derde hoofdstuk uit hematologie van Anneleen Cottyn

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2021/2022 Written for academic year
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Hogeschool West-Vlaanderen (HoWest)
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MLT03 - Werken In Een Hemato-klinisch Lab (MLT03)
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Zeer gemakkelijk deel uit de cursus, alles over de elektroforese met voorbeelden bij

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3 Hemoglobine elektroforese
Hemoglobinescreening
▪ Soorten: Capillarys, Capillarys Flex Piercing, Minicap, Minicap Flex Piercing
▪ Flex piercing: voor veiligheid, andere implementaties hoe je in het labo werkt
Parameter Capillarys Capillayrs Minicap Minicap Flex
Flex Piercing Piercing
Staal Veel stalen Veel stalen Weinig stalen Weinig stalen
RBC Volbloed RBC Volbloed
250 µL 1 mL 250 µL 1 mL
In toestel + hemolyse-opl + hemolyse-opl + hemolyse-opl + hemolyse-opl
Open buizen in toestel Op carrousel Tubes met dop
Buis met dop Carrousel met
adaptors
# capillairen 8 2 8 2
Controle Aan het begin van elke analysereeks + om de 10 rekjes
Normale HbA2-controle (gelyofiliseerd)
→ met barcode: NORMAL-CTL
→ zonder barcode: SAMPLE

Hb op capillaire elektroforese
Scheiding van de verschillende fracties in de capillairen op basis van lading
Detectie bij 415 nm
Hb-fracties bij normale patiënt

Naam type Hb % in volwassene Samenstelling ketens Component
HbA 96-98 𝛼2 𝛽2 Major component
HbA2 2,3-3,5 𝛼2 𝛿2 Mino component
HbF <2 𝛼2 𝛾2 Foetaal Hb
Normale patiënt:
Connected book
image
E. Ten Boekel, B.A. de Boer Heron-reeks - Klinische chemie en hematologie voor analisten 1
Edition: Unknown ISBN: 9789491764516 Edition: 3

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