ASCP MB: Extra-Practice Questions and Answers Scored A

ASCP MB: Extra-Practice Questions and Answers Scored A The Klenow fragment of E. coli DNA polymerase I is used: - Answer ️️ -to label DNA probes by random primer labeling. What is a solution hybridization method for the detection of nucleic acid:protein interactions? - Answer ️️ -gel mobility shift assay _____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific interaction occurs, visible with an apparent increase in molecular size on a gel due to the protein:nucleic acid interaction. - Answer ️️ -Gel mobility shift assay Which probe is most often used to detect trisomy 21 by interphase FISH? - Answer ️️ -alpha satellite DNA probe _____ detect specific chromosomes and numbers of the specific chromosomes. - Answer ️️ - Alpha satellite DNA probe Which reagent generates a signal during the annealing stage of a quantitative real time PCR reaction? - Answer ️️ -molecular beacon Methylation of cytosine residues is involved in the alteration of gene expression in a number of cancers. Which sequencing technique is best suited to determine if methylation of cytosine residues has taken place in the promoter region of a given gene? - Answer ️️ -bisulfite sequencing _____ is a modified chain terminator method specifically designed to detect methylated nucleotides. - Answer ️️ -Bisulfite sequencing Which method is best suited to screen for mutations in a gene having multiple possible mutations? - Answer ️️ -single strand conformational polymorphism (SSCP) _____ is a screening test in which the exact nature of a mutation does not need to be known in order to detect the presence of a mutation. - Answer ️️ -Single strand conformational polymorphism (SSCP) What is the best method to rule out the possibility of genomic DNA giving a false-positive result in an RT-PCR reaction? - Answer ️️ -use of upstream and downstream primers that span an exon-intron-exon region of the target *amplicaons generated from genomic DNA would be larger than the specific amplicon generated from a mature, spliced mRNA A common use for pulsed-field gel electrophoresis is: - Answer ️️ -epidemiological typing of bacterial strains. _____ after digestion of bacterial chromosomes with restriction enzymes is used to determine similarity between bacterial isolates. - Answer ️️ -PFGE A translocation in which chromosome pair creates a BCR/ABL1 fusion gene product associated with CML? a. 11;18 b. 14;18 c. 9;22 d. 9;14 - Answer ️️ -t(9;22) The presence of a distinct band along with the smear indicates at least 1% of the patient's B cells have a _____ of the Ig heavy chain. - Answer ️️ -monoclonal r