Molecular Diagnostics Exam

Molecular Diagnostics Exam Which of the following determines the sensitivity of an immunoassay? a. avidity of the antibody b. specificity of the antibody c. affinity of the antibody e. none of the above - answerc. affinity of the antibody Monoclonal antibodies provide immunoassays: a. low initial costs b. broad reactivity c. shorter lead times d. lot to lot consistency - answerd. lot to lot consistency Which of the following characterizes a capture enzyme assay? a. best for high-molecular weight substances b. requires no separation step c. requires two wash steps d. more sensitive than heterogenous enzyme assays - answerc. requires two wash steps Which of the following is true of a fluorescence polarization immunoassay? a. the amount of fluorescence is directly proportional to concentration of the analyte b. large molecules polarize more light than smaller molecules c. both antigen and antibody are labeled d. when binding occurs, there is quenching of the fluorescent tag - answerb. large molecules polarize more light than smaller molecules In an immunoassay, serum is added to a microtitier well coated with specific antibody. After incubating and washing, enzyme-labeled specific antibody is added. This procedure is: a. homogeneous immunoassay b. a sandwich immunoassay c. a competitive binding immunoassy d. an enzyme-multiplied immunoassay - answerb. a sandwich immunoas...