VCU Biochem Exam 2 Questions And Answers

VCU Biochem Exam 2 Questions And Answers is binding of ligands reversible? - ANS-yes, it is essential for it to be reversible cooperativity - ANS-In multisubunit proteins, conformational changes in one subunit can affect the others functions of globular proteins - ANS-1. storage of ions and molecules (myoglobin and ferritin) 2. transport of ions and molecules (hemoglobin, serotonin transporter) 3. defense against pathogens (antibodies, cytokines) 4. muscle contraction (actin, myosin) 5. biological catalysis (chymotrypsin, lysozyme) ligand - ANS-a molecule that binds to a protein (typically a small molecule) binding site - ANS-region in the protein where the ligand binds (ligand binds with noncovalent forces) what does the fraction of bound sites depend on? - ANS-1. the free ligand concentration (known) 2. Kd (determined graphically or least-squares regression) what is binding expressed in terms of when ligand is a gas - ANS-partial pressure Kd (dissociation constant) - ANS-[P][L]/[PL] Theta (fraction of occupied binding sites) - ANS-[L]/ ([L] + [Kd])...