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BIOCHEM C785 pre assessment #3

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Amino Acids RNA nucleotides DNA polymerase Correct! In PCR, a DNA sample is separated into single strands and incubated with DNA polymerase, deoxynucleotides (dNTPs), and two short DNA primers whose sequences flank the DNA segment of interest and thus define the region to be amplified. Recall from the section on DNA replication that DNA polymerase needs a primer to begin DNA synthesis. This requirement means the primers will direct the DNA polymerase to only synthesize complementary strands of the target DNA. DNA nucleotides Correct! In PCR, a DNA sample is separated into single strands and incubated with DNA polymerase, deoxynucleotides (dNTPs), and two short DNA primers whose sequences flank the DNA segment of interest and thus define the region to be amplified. Recall from the section on DNA replication that DNA polymerase needs a primer to begin DNA synthesis. This requirement means the primers will direct the DNA polymerase to only synthesize complementary strands of the target DNA. Template DNA Correct! In PCR, a DNA sample is separated into single strands and incubated with DNA polymerase, deoxynucleotides (dNTPs), and two short DNA primers whose sequences flank the DNA segment of interest and thus define the region to be amplified. Recall from the section on DNA replication that DNA polymerase needs a primer to begin DNA synthesis. This requirement means the primers will direct the DNA polymerase to only synthesize complementary strands of the target DNA. DNA Primers Correct! In PCR, a DNA sample is separated into single strands and incubated with DNA polymerase, deoxynucleotides (dNTPs), and two short DNA primers whose sequences flank the DNA segment of interest and thus define the region to be amplified. Recall from the section on DNA replication that DNA polymerase needs a primer to begin DNA synthesis. This requirement means the primers will direct the DNA polymerase to only synthesize complementary strands of the target DNA. Ribosomes RNA Primers Correct answers: DNA polymerase,DNA nucleotides,Template DNA,DNA Primers 16 8 32 The correct answer is 32. 2x2x2x2x2=32 10 Correct answers: 32 5’ UAG AGC CAU 3’ 5' UAC AGC CAU 3' 5’ UAC AGC CAC 3’ Correct! The correct answer is 5' UAC AGC CAC 3', which also gives Tyr Ser His 5’ UAC AGC CAA 3’ Correct answers: 5’ UAC AGC CAC 3’ Silent Missense The correct answer is missense mutation. The nucleotide sequence changes, and one amino acid is changed. Since the third codon is changed from GGA to CGA, this is a point mutation. If the point mutation results in an amino acid change compared to the original sequence, the point mutation is a missense mutation. Nonsense Insertion Correct answers: Missense True The correct answer is Option 1 because an autosomal dominant disorder would be inherited on numbered chromosomes, not sex chromosomes X or Y. Also, at least one dominant allele (yellow box) needs to be prese

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