Histotechnology: A Self-Instructional Text, 4th ed correctly answered graded A+

Histotechnology: A Self-Instructional Text, 4th edFor adequate fixation, the fixative volume should be at least ____ times greater than the tissue volume. A. Equal B. 5-10 C. 10-14 D. 15-20 - correct answers 15-20 This type of microscope is used for the identification of crystals as well as to make identification of amyloid A. Light microscope B. Polarizing microscope C. Phase-contrast microscope D. Darkfield microscope - correct answers Polarizing microscope For most routine H & E staining, the sections should be 1 cell layer thick, and should be cut at ____ micrometers. A. 1-2 B. 2 C. 3-4 D. 6 - correct answers 3-4, 4-5 10% neutral buffered formalin is the most widely used solution for routine formalin fixation and has a pH of ____. A. 2.2 B. 4.5 C. 6.8 D. 9.0 - correct answers 6.8 __________ is unique in that it is the only substance used in histotechnology both as a fixative ingredient and as a stain. A. Picric acid B. Osmium tetroxide C. Potassium dichromate D. B-5 - correct answers Picric acid Coagulant fixative, strong coagulant of nucleoprotein but leaves DNA soluble Which of the following is used in the dehydration of tissue? A. Ethyl alcohol B. Methyl alcohol C. Isopropyl alcohol D. All of the above - correct answers All of the above If the blade has too little of a tilt when cutting sections on the microtome, this issue can occur: A. Holes in sections B. Failure of ribbon to form C. Lengthwise scratches in the ribbon D. Varied thickness of sections (thick and thin) - correct answers Varied thickness of sections (thick and thin) - 30˚ Too much tilt: scraped rather than cut sections Carpal tunnel syndrome, trigger finger, tendonitis and thoracic outlet compression syndrome are all examples that result from this type of hazard: A. Biological or infectious B. Mechanical C. Chemical D. Fire - correct answers Mechanical Creutzfeldt-Jakob disease is a prion disease affecting primarily nervous tissue. The prions are extremely resistant unlike many other biological and infectious hazards. They are however susceptible to: A. 1 hour concentrated formic acid fixation B. 10% non-buffered formalin fixation C. Freezing D. Drying - correct answers 1 hour concentrated formic acid fixation and 5% sodium hypochlorite for 2 hours Autoclaving for 1 hour at 121˚C/132-134˚C and 2kg/cm2 Electrical fires that must be extinguished with nonconductive media are classified as ____ fires. A. Class A B. Class B C. Class C D. Class D - correct answers Class C Class A - Combustible materials: wood, plastics, paper, textiles Class B - Flammable liquids and gases Class D - Combustible and reactive elements