Histotechnology: A Self-Instructional Text fully solved graded A+ 2023/2024

Histotechnology: A Self-Instructional TextFor adequate fixation, the fixative volume should be at least ______ times greater than the tissue volume. - correct answers 15-20 This type of microscope is used for the identification of crystals as well as to make identification of amyloid. - correct answers Polarizing microscope For most routine H & E staining, the sections should be 1 cell layer thick, and should be cut at ______ micrometers. - correct answers 3-4 10% neutral buffered formalin is the most widely used solution for routine formalin fixation and has a pH of ____. - correct answers 6.8 __________ is unique in that it is the only substance used in histotechnology both as a fixative ingredient and as a stain. - correct answers Picric acid Which of the following is used in the dehydration of tissue? A. Ethyl alcohol B. Methyl alcohol C. Isopropyl alcohol D. All of the above - correct answers All of the above If the blade has too little of a tilt when cutting sections on the microtome, this issue can occur: - correct answers Varied thickness of sections (thick and thin) Carpal tunnel syndrome, trigger finger, tendonitis and thoracic outlet compression syndrome are all examples that result from this type of hazard: - correct answers Mechanical Creutzfeldt-Jakob disease is a prion disease affecting primarily nervous tissue. The prions are extremely resistant unlike many other biological and infectious hazards. They are however susceptible to: - correct answers 1 hour concentrated formic acid fixation Electrical fires that must be extinguished with nonconductive media are classified as________ fires. - correct answers Class C The National Fire Protection Association (NFPA) chemical hazard label consists of a diamond with four color coded boxes- each with numerical values from 0 to 4 to rate each hazard. What would a three in the blue box indicate? - correct answers Severe health hazard According to the NFPA chemical hazard label, what would a zero in the red box indicate? - correct answers No fire hazard Which of the following errors can lead to pale nuclear staining on an H&E stained slide? - correct answers Slide stained with overoxidized hematoxylin Green birefringence following _____ staining is considered the most specific technique for the demonstration of amyloid. - correct answers Congo red Cells found in connective tissue which contain abundant secretory granules including histamine and heparin. - correct answers Mast cells These two stains are used to demonstrate lipids in tissue: - correct answers Sudan black B and Oil red O ______are small cells that function in the CNS to produce, and probably maintain, the myelin sheath surrounding many axons. They are the most numerous of the glial cells and are found in both the gray and white matter. - correct answers Oligodendroglia Protein-coated genes that need living cells to provide energy and the machinery for duplication. - correct answers Viruses