Histotechnology Ch 3 - Instrumentation questions and answers graded A+ 2023/2024

Histotechnology Ch 3 - InstrumentationEffect of too cold instrumentation - correct answers tissue fragments/splits Effect of too warm instrumentation - correct answers tissue collects at blade edge What is the proper temperature for cold instruments? - correct answers -20 for normal tissue -10 for brain, liver, spleen, LN, endometrial scrapings What type of microscope? Anisotropic or five fringant material will be bright against a dark background at the optical paths of the polarizer and analyzer are crossed - correct answers Polarizing What type of microscope? Used on unstained specimen allowing transparent objs to be seen clearly - correct answers phase-contrast What type of microscope? Directly transmitted light excluded and only scattered or oblique light used - correct answers darkfield What type of microscope? Optical phenomenon in which light of 1 wavelength absorbed bu substance and instantly readmitted as light of longer wavelength - correct answers fluorescence What type of microscope? Magnification range from 100-500,000 diameters, obtains extra resolving power by replacing ordinary light source of LM w/ electron gun, electrified tungsten filament that emits electrons - correct answers EM What type of microscope? Specimen either transmits or deflects electrons; 2D image; useful for muscle and kidney dx - correct answers transmission EM What type of microscope? Dramatic 3D image; electron beam sweeps surface of specimen and releases secondary electrons used to study surfaces of specimen; used to study cell surface membrane changes and research - correct answers scanning EM What type of LM lens is used for: x2.5-4 magnification - correct answers scanning lens What type of LM lens is used for: x10-20 magnification - correct answers intermediate lens What type of LM lens is used for: x40-50 magnification - correct answers high powered dry lens What type of LM lens is used for: x90-100 magnification - correct answers oil immersion lens What is the equation for determining total magnification? - correct answers ocular magnification X objective magnification = total -ratio of virtual image to the size of the obj as it appears to unaided eye at distance of 10" Angle formed by the intersection of the block face and adjacent cutting facet of the knife - correct answers clearance angle What should the clearance angle be? - correct answers 3-8 degrees Angle made when the 2 cutting facets of a microtome knife intersect - correct answers bevel angle What should the bevel angle be? - correct answers most american knives = 28-32 degrees The optical ability to distinguish 2 objs at a min distance apart as 2 objs; objs closer than 0.2 um viewed as 1 - correct answers Resolution Eye piece of the microscope or lens found at the upper end of the barrel or body tube - correct answers ocular piece the lens at the lower end of the body tube; provides magnification and resolution - correct answers objective Measurement of minute distances w/in microscope - correct answers micometry Cutting a thin section of tissue for microscopic exam - correct answers microtomy