MLPAO Exam Questions and Answers Already Passed

MLPAO Exam Questions and Answers Already Passed Blood Smears should be prepared from? EDTA Blood The vacutainer collected for most haematology tests? Lavender Test that measure the oxygen carrying ability of the blood is? Hemaglobin Test that detects abnormal hemaglobin? Hemoglobin Electrophoresis What is RBC indices? measurement of the total RBC count as well as size, weight, and hemoglobin concentration of the RBC's What is the test that determines the different proportions of leukocytes? WBC Differential Test that indicates th enumber of thrombocytes in a L of Blood? Platelet Count Test that counts the # of immature RBC's? Reticulocyte Count What test helps diagnose hemophilia? Factor VIII ? Factor VIII deficiency contributes to what disease? Hemophilia A What test is used for patients undergoing anticoagulant therapy? Prothrombin Time What colour tubes are collected for a WBC count and an APTT? Light Blue and Lavender Blood films stained with Wright's stain are not prefixed because? Methanol in the stain does the fixing A __________ test will be increased in patients with arthritis or inflammation Erythrocyte sedimentation Rate Why is blood for microhematocrit thoroughly mixed? Ensure even distribution of all blood components What are the components of whole blood? Plasma Buffy coat and Erythrocytes What kind of media is Chocolate agar? Enriched What is a SAB plate selective for? Fungi -- Yeast What temperature is a SAB plate incubated at? 30C What is the difference between beta, alpha, and gamma Hemolysis? Beta: Complete hemolysis -- yellow Halo Alpha: Partial Hemolysis -- Green Halo Gamma: No Hemolysis -- nothing What bacteria causes beta hemolysis? Strep and Staph What test is done to differentiate between strep and Staph? Biochemical test What is ZN stain? Ziehl-Neelson Stain: Acid-fast stain What microbes must be ZN stained? Acid-fast - resist decolourization by acid alcohol due to waxy coating Ex. Mycobacteria What colour will AFB stain (ZN STAIN)? Red What is the kinyoun Method? Cold version of ZN stain Carbol Fuschin red is mixed with a phenol to replace heat fixing What are bacterial Spores? A dormant form of bacterial material Resistant to temp, chemicals, radiation, and dessication What causes Malaria? Plasmodium spp. What is the basic additive in all media? Agar and carbohydrates What is CLED media used for? Differential plate for Urine What media is used for lactose fermenters? MAC What are the gram staining Steps? a suction device used to suck up liquids is a : safety bulb the pipette with bulged out portion in the middle is volumetric pipette which durable material is used to make heat resistant glassware borosilicate for small loads, the autoclave is set at: 121 degrees c for 15min at 15 psi a TC pipette is rinsed out after delivery which type of plasticware can be autoclaved polyethylene what is the autoclave used for sterilization why is it important for all air to be removed from the autoclave during sterlization air reduces the temperature in the autoclave which of these is used to measure the RPM of a centrifuge tachometer which microscope would be best to examine an unstained, wet preparation of cells before dehydration phase contrast heat labile reagents must be slowly warmed to 37 degrees C commercial formaldehyde is usually 40% formaldehyde gas in water while running coagulation tests on a photo optical instrument the prothrombin time results for a bath of patient samples are consistently prolonged but the partial thromboplastin time results for the patient samples are within normal limits the lab technician should check the level of thromboplastin in the reagent walls which water purification method involves boiling water and then letting the steam cool distillation what is the total magnification when using a 15X ocular lens and a 25X objective lens on a light microscope 375X 15x25=375 super heating steam in an autoclave diminishes its sterilizing capacity