BACE EXAM 4.0 LABORATORY SKILLS 100% CORRECT

BACE EXAM 4.0 LABORATORY SKILLS 100% CORRECT the process of cleaning something, especially with a chemical, in order to destroy bacteria. Disinfection Decontamination is the removal of dangerous substances, radioactivity or germs from...? an area object or person Decontamination is the removal of what? dangerous substances radioactivity and germs What is the purpose of aseptic technique? Aseptic technique assures that you don't introduce contaminants into a specimen and that infectious agents are not spread to you or laboratory surfaces When do technicians practice aseptic technique? Technicians practice aseptic technique when culturing cells Why do technicians practice aseptic technique? to ensure they are growing pure cultures of their desired cells. When transferring bacteria using aseptic technique, it is common practice to do what? to place the sterile loop into the culture tube by moving the tube over the loop When transferring bacteria using aseptic technique, it is common practice to place the sterile loop into the culture tube by moving the tube over the loop, rather than what? rather than moving the loop into the tube. destroys all microorganisms Sterilization the neutralization or removal of dangerous substances, radioactivity, or germs from an area, object, or person. Decontamination What should not be used to disinfect an area that is potentially contaminated by DNA? Alcohol When using a microscope on the highest power objective, you should use....? the fine focus knob Objectives are located beneath the stage bright field microscope Light source is located above the specimen inverted microscope Uses a laser light to scan samples that have been dyed Confocal microscope Uses electrons to scan samples in near-vacuum conditions Electron Microscope A solution with a pH of 3.0 is how many times more acidic than a solution with a pH of 6.0? 1,000 The pH scale is ______ logarithmic How are enzyme solutions always prepared? using a buffered solvent at a specific pH Why are enzyme solutions always prepared using a buffered solvent at a specific pH? because a buffered solvent is resistant to fluctuations in pH Why are buffered solvents resistant to fluctuations in pH? because it is made with a combination of a weak acid and its conjugate base Enzymes best retain their function within a ______ range? narrow pH range DNA is purified using what? anion exchange column chromatography How is DNA purified using anion exchange column chromatography? because DNA is negatively charged, binds to the positively charged resin, and is eluted with increasing salt. Evaluate the appropriateness of using the potential primer (below the DNA strand) to amplify the specific sequence of DNA. • DNA Sequence of interest: 3'-ACATGCTGCTGCTGGTCACAAGTCAATTCCTAA-5' • Potential