Biotechnician Assistant Credentialing Practice Exam Latest Update Graded A

Biotechnician Assistant Credentialing Practice Exam Latest Update Graded A After performing the PCR reaction with the primers she designed to amplify lanA, the same technician found that her PCR reaction contained two distinct bands, one at the expected size of 1200 kilobase pairs, and another unexpected band at 2000 kilobase pairs. What is the most likely reason there is an unexpected band in her PCR reaction? She used an annealing temperature that was too low In a biotechnology lab, tap water is best used for: The first rinse when washing glassware The order of amino acids on a polypeptide chain is encoded by: mRNA How do DNA molecules vary from one species to another? The number of genes and non-coding regions What is agar? Solid media on which bacteria grow What part of amino acid differs from one another? R group How are the proteins in fractions collected during column chromatography visualized? Using polyacrylamide gel electrophoresis (SDS PAGE) Which of the following is an mRNA codon? UAU What is the purpose of SDS when used in SDS PAGE electrophoresis? Denature the proteins and give them a positive charge Which department is in charge of testing a biopharmaceutical product to ensure its safety and potency? Quality Control (QC) Lipids provide which of the following functions for cells? Stored energy Which of the following is a monosaccharide commonly used as an energy source for cells in cell culture media? Glucose Where does translation occur in prokaryotes? At the ribosomes In aerobic respiration carbohydrates are ultimately broken down into: CO2, H20, ATP Most ATP in eukaryotic cells is produced in the Mitochondria An aqueous solution and yields a hydrogen ion when ionized (Definition) Acid Defined as "the moles of solute per liter of solution" Molarity A solution that resists changes in pH Buffer A measurement of the concentration of hydrogen ion in a sample pH Type of test used to measure the amount of total protein present Concentration assays Type of test used to measure the amount of active ingredient in a drug Potency assays Type of test that demonstrates whether or not a specific enzyme or drug is having the desired effect for which it was designed Activity assays A highly specific test to determine protein concentration based on a particular antigen-antibody interaction ELISA A document listing the detailed steps of key processes to help ensure consistent and high quality output SOP (Standard Operating Procedure) Viscous buffer used to track DNA or proteins during electrophoresis Loading Dye A document used in Biomanufacturing with step-by-step instructions of the production process, and spaces to record information during the production process Batch Record Quantitative measurement of absorbance based on visible light, near-ultraviolet, and near-infrared light sources passing through a solution Spectophotometry The use of an electric field to separate DNA in agarose Gel electrophoresis The process of making RNA from DNA Transcription The process by which RNA is decoded and polypeptides are formed Translation Autonomously replicating small circles of DNA presenting in some bacteria Plasmids The deliberate modification of the characteristics of an organism by manipulating its genetic material Genetic engineering Parkinson's, diabetes, and cystic fibrosis (CF) are examples of Genetic disorders Organism that does not have nucleus or membrane bound organelle, so its DNA is contained in the cytoplasm Prokaryotes Organism containing a nucleus and other organelles enclosed within membranes Eukaryote A process of correcting mutations which cause genetic diseases and disorders by delivering therapeutic DNA into a patient's cells Gene Therapy The use of living systems or organisms to develop or make useful products Biotechnology The deliberate linkage of DNA from different sources into one molecule Recombinant DNA What is the formula for Dilution of Stock Solutions? C1V1=C2V2 (T/F) Cells produced by mitosis are haploid False (T/F) Germinating or sprouting plants are actively undergoing mitosis. True (T/F) Meiosis occurs in all sexually reproducing eukaryotes True (T/F) Meiosis is a type of cell division which increases the chromosome number by two False (T/F) During meiosis, chromosomes may "cross-over" and shuffle their genetic information True (T/F) Plant breeders can take advantage of meiosis by selective breeding: pollen from plants with desired traits are purposely crossed with other plants exhibiting desired traits. True (T/F) DNA replication occurs 3' to 5'. False (T/F) DNA polymerases are the enzymes that create DNA molecules by assembling nucleotides True (T/F) DNA polymerases from thermophiles (heat-loving bacteria) are used in polymerase chain reaction (PCR) True (T/F) DNA is replicated on the leading and the lagging strands in exactly the same way False The Food and Drug Administration (FDA) requires biopharmaceutical companies to follow current good manufacturing practices (cGMPs) (True or False) True Which of the following is not a document used in the manufacture of a biopharmaceutical product? Batch Recipe Which department in a biopharmaceutical company has final say if a production batch may be released for sale or use? Quality Assurance Which department in a biopharmaceutical company is responsible for testing a product to ensure it is safe and efficacious? Quality Control Which department in a biopharmaceutical company is responsible for identifying future products? Research and Development The study of decision-making as it applies to moral decisions regarding advances in biotechnology is called bioethics (True or False) True Food and Drug Administration (FDA) regulations can be found in? The Code of Federal Regulations Hypothesis A tentative statement about the natural world leading to deductions that can be tested. If the deductions are verified, the hypothesis is provisionally corroborated. If the deductions are incorrect, the original hypothesis is proved false and must be abandoned or modified. Hypotheses can be used to build more complex inferences and explanations. Theory In science, a well-sustained explanation of some aspect of the natural world that can incorporate facts, laws, inferences, and tested hypotheses Law A descriptive generalization about how some aspects of the natural world behaves under stated circumstances