MLPAO Test Prep ~ Hematology questions and answers latest top score.

MLPAO Test Prep ~ Hematology questions and answers latest top score. What does CBC stand for? - correct answers.Complete Blood Count What are the formed elements of blood? - correct answers.WBC, RBC, and Platelets EDTA Anticoagulant - correct answers.Maintains cell shape and size and prevents blood from clotting by binding to calcium Heparin Anticoagulant - correct answers.Initiates cell clumping and provides blue background staining Citrate Anticoagulant - correct answers.Dilutes the blood due to the volume of the liquid and is used for platelet count only CBC handling and storage: Refrigerate at __ᵒC until testing - correct answers.4 CBC handling and storage: When transporting to other labs/hospitals - correct und with ice packs CBC handling and storage: Samples are _____ before testing - correct well by inversion CBC handling and storage: Samples must be tested within __ - correct answers.24h CBC handling and storage: Peripheral blood smears must be made within - correct answers.24h CBC handling and storage: Samples kept for longer than ___ = CANNOT BE USED FOR TESTING - correct answers.24h What happens if CBC is tested after 24h? - correct answers.Platelets clump, WBC nuclei disintegrate, WBC cytoplasm contain dark granules and Vacuoles, and RBCs swell and break down Coagulation: Specimen of choice? - correct answers.Plasma Coagulation: What colour tube? What is the additive? - correct blue, sodium citrate Sodium Citrate binds to ___ preventing ____ - correct um, coagulation Sodium Citrate tube must be - correct d to the maximum draw capacity Coagulation: Document if patient on - correct oagulant therapy or aspirin Handling and Storage of Coagulation samples: Preparation requirements - correct answers.Centrifuge and separate Handling and Storage of Coagulation samples: ____ plasma if not tested within ___ of arrival - correct answers.Freeze, 4h Coagulation samples: Some clotting factors are considered to be - correct y labile (destroyed at room temperature) What are the three methods of Peripheral blood smears (PBS)? - correct answers.Wedge method (manual), Spun method (automated slide spinner), and automated method (analyzer) Thickness of film: HEMOGLOBIN/HEMATOCRIT INCREASED = angle of the spreader slide ___ - correct answers.Decreased Sodium Citrate tubes should be drawn ___ except when blood cultures are ordered. - correct answers.First Thickness of film: HEMOGLOBIN/HEMATOCRIT DECREASED = angle of the spreader slide ___ - correct answers.Increased HCT Values: Male, Female - correct answers.0.40-0.50, 0.35-0.59 Good blood film should be ___ to ___ the length of the slide - correct answers.2/3, ¾ Good blood film should have a slightly ___ or ____ edge - correct answers.Rounded, feathered The angle of the spreader slide is approximately ___ degrees - correct answers.35 The greater the angle of the spreader slide the ____ and _____ the film - correct answers.Shorter, Thicker The lower the angle of the spreader slide the ___ and ____ the film - correct answers.Longer and Thinner Blood film should be allowed to ____ ___ completely before staining procedures - correct dry Fixative found in stain is - correct answers.Methanol Slides are washed with buffer of pH ___ to develop colour on the slide - correct answers.6.4 Stain is comprised of ____ and ____ dyes - correct answers.Basic, acidic What is the basic (cationic) dye? - correct answers.Methylene blue Methylene blue stains the ___ of the cell and the granules of _____ (____ in colour) - correct answers.Nucleus, basophils, blue-grey What is the acidic (anionic) dye? - correct answers.Eosin Eosin stains ___ and the granules of ____ (_____ in colour) - correct answers.Hemoglobin, eosinophils, red-orange Neutral cell components utilized ___ dye resulting in the stain to be ____ - correct answers.Both, Purple Manual staining procedure: Stain for approximately ___ - correct answers.1-3m Manual staining procedures: Add ___ proportions of buffer - correct Manual staining procedures: Allow slide to sit for at least ___ - correct answers.3m What to do if slides are too blue? - correct answers.Decrease stain time, increase rinsing time, decrease buffer pH What to do if slides are too pink? - correct answers.Increase stain time, decrease rinsing time, increase buffer pH What may cause slides to have stain deposits? - correct answers.Dirty slide, stain solutions not filtered properly, stain dried up on slide. HCT stands for? - correct answers.Hematocrit Hematocrit is the volume of RBCs after centrifugation for ____ at ____ rpm - correct answers.5m, 1200 HCT: Cells are typically ___% of blood volume - correct answers.40-50 HCT: Must be centrifuged within ___ of collection - correct answers.4h HCT Normal Values: Critical - correct answers.0.20 and 0.60 HCT Normal Values: Infant - correct answers.0.50-0.60 HCT Normal Values: Toddler - correct answers.0.35-0.42 HCT Normal Values: Male - correct answers.0.40-0.50 HCT Normal Values: Female - correct answers.0.35-0.59 Optical light scatter is used in - correct answers.Blood cell differentials (counting) Optical light scatter: Forward angle light scatter measures - correct answers.Cell size and volume Optical light scatter: Side light scatter measures - correct larity and nuclear size Units: RBC count - correct answers.x10^12/L Units: WBC count - correct answers.x10^9/L Units: Platelet count - correct answers.x10^9/L Units: Reticulocytes - correct answers.Whole number Units: Hemoglobin - correct answers.g/L Units: Hematocrit - correct answers.L/L Units: MCH - correct Units: MCHC - correct answers.g/dL MCH stands for - correct answers.Mean corpuscular hemoglobin MCHC stands for - correct answers.Mean corpuscular hemoglobin concentration The difference between MCH and MCHC is that the MCHC measurement - correct answers.Takes the volume or size of the red blood cell into account. Directly measured tests include - correct answers.RBC, WBC, Platelet, Reticulocyte, and Hemoglobin Calculated tests include - correct answers.Hematocrit, MCH, MCHC HCT Errors: Improper sealing of capillary tube will cause falsely increased or decreased HCT? - correct answers.Decreased (loss of RBCs) HCT Errors: Improper mixing of blood will cause falsely increased or decreased HCT? - correct answers.Either HCT Errors: Increased concentration of additive in tube will cause falsely increased or decreased HCT? - correct answers.Decreased (under filled tube) HCT Errors: Centrifuge speed and timer will cause falsely increased or decreased HCT? - correct answers.Increased (trapped plasma) HCT Errors: Improper collection of specimen will cause falsely increased or decreased HCT? - correct answers.Either HCT Errors: Hemolysis will cause falsely increased or decreased HCT? - correct answers.Decreased (breakdown of RBCs)