Next Generation Sequencing Exam Questions And Answers 100% Correct

Next Generation Sequencing Exam Questions And Answers 100% Correct Genomics - ANSWER- study of the whole genome Transcriptomics - ANSWER- study of the produced RNA Next generation sequencing - ANSWER- group of automated techniques used for rapid DNA sequencing dideoxy sequencing - ANSWER- a method of DNA sequencing that uses dideoxyribonucleotides to terminate the growth of DNA strands discovered by Fredrick Sanger chemical sequencing - ANSWER- DNA subject to controlled chemical treatments that generate breaks of the molecules at specific nucleotides chain termination sequencing - ANSWER- DNA sequencing by polymerization in the presence of dideoxynucleotide triphosphate as DNA chain terminators chain terminators - ANSWER- a dideoxynucleotide which lacks the 3' hydroxyl group and the 2' hydroxyl group insertion of chain terminators - ANSWER- strand extension is prevented what does chain termination sequencing require - ANSWER- template and oligonucleotide primer for DNA polymerase and a pool of normal nucleotides containing a small amount of one dideoxyribonucleoside triphosphate procedure of Sanger sequencing - ANSWER- the insertion of the dideoxyribonucleotode blocks the growth of the molecule to determine the position of the nucleotide added with the fragment., therefore all 4 dideoxiribonucleotides must be added in different test tubes, then loaded on polyacrylamide to differentiate molecules which differs in nucleotides direction in which sequences are red on polyacramide gel - ANSWER- bottom up dye dideoxy terminators - ANSWER- fluorescently labelled chain terminators which allow the addition of all dideoxyribonucleotides to the same test tube advancement of Sanger sequencing - ANSWER- radioactive polyacrylamide slab gel, AB slab gel sequencing, and AB capillary sequencing