TSI Tests (Triple Sugar Iron) Questions With Complete Solutions

What does TSI mean? correct answer: Triple Sugar Iron What is a TSI (Triple Sugar Iron) Test also referred to as? correct answer: Kligers Iron Test What does a TSI Test, test for? correct answer: Bacteria's ability to: -Ferment glucose and lactose/sucrose -Produce gas -Reduce Sulfur What are the ingredients of this media? correct answer: Glucose, Sucrose, and Lactose What is the ratio of Glucose, Sucrose, and Lactose in this media? correct answer: 1:10:10 What is the sulfur source in a TSI Test? correct answer: Sodium Thiosulfate What is the fermentation indicator in a TSI Test? correct answer: Phenol Red What is the hydrogen sulfide indicator in a TSI Test? correct answer: Ferrous Sulfate What are the 2 steps to inoculating this media? correct answer: 1.) Stab the butt 2.) Fishtail the slant In the Fermentation of sugars portion of a TSI test, which sugar is limited, and why? correct answer: Glucose, because it is bacteria's primary source of carbon Which sugar will bacteria always try to ferment first? correct answer: Glucose