MIBO 3510 L exam 1 2023 with 100% correct answers

glucose What should you subtract form media if you want to grow photolithoautotrophs? Pseudomonas organism that denitrifies and returns N2 to the atmosphere rhizobium organism in the soil that fixes nitrogen into NH3/NH4+ nitrosomonas organism that oxidizes NH4+ into NO2- nitrobacter organism that oxidizes NO2- into NO3- take out nitrogen What should you subtract from media if you want to grow nitrogen fixing bacteria? What is biosafety level 1? Well-characterized agents which do not cause disease in healthy humans What is BSL2? Moderate potential hazard to humans and the environment (cause mild disease and/or are difficult to contract in the laboratory) What is BSL3? Can cause serious and potentially lethal disease via inhalation What is BSL4? Agents that are easily transmitted via aerosols and cause severe to fatal disease for which there are no available vaccines or treatments sterile guaranteed free of live organisms/viruses aseptic Only contains the organisms you want culture Collection of live organisms grown in a lab pure culture Culture known to contain only one organism (started from 1 cell) What causes bacteria to have different morphologies? -Growth rate (size) -Motility (rate of spread) -Pigment production -Cell wall comp. 2 energy sources for metabolic classification 1. light (photo) 2. chemicals (chemo) 2 electron sources for metabolic classification 1. organic (organo) 2. inorganic (litho) i.e. NH3,H2 2 carbon sources for metabolic classification 1. organic (hetero) i.e. sugars 2. CO2 (auto)