First Aid USMLE Step 1: Pharmacology 2023

First Aid USMLE Step 1: Pharmacology 2023 In the Michaelis-Menten kinetics what do the following mean: Km Vmax Draw a typical hyperbolic curve on a Michaelis-Menten Plot, then draw how the addition of a competitive and non-competitive inhibitor would change the kinetics of the reaction. - ANS-*In the Michaelis-Menten kinetics what do the following mean:* Km: - the concentration of substrate at 1/2 Vmax - this is INVERSELY related to the affinity of enzyme for its substrate Vmax: - maximal velocity at which enzymatic reaction occurs - is directly proportional to the enzyme concentration Competitive inhibitor: would raise Km but Vmax is the same Non-competitive inhibitor: would lower Vmax? Draw a Lineweaver-Burke plot. What information do the following intercept points correspond to? X axis Y axis slope y intercept x intercept