(MB) ASCP EXAM STUDY GUIDE QUESTIONS AND ANSWERS 2023 SOLUTION COMPLETE 100% VERIFIED GUIDE.

(MB) ASCP EXAM STUDY GUIDE QUESTIONS AND ANSWERS 2023 SOLUTION COMPLETE 100% VERIFIED GUIDE. What kind of bond do histones have with DNA? - Answer - Ionic What amino acids compose histones? - Answer - Lysine and Arginine Which lymphoma has t-cell rearrangements? - Answer - Sezary Syndrome Acute Promyelocytic leukemia translocation? - Answer - t(15,17) Leading strand polymerase: - Answer - Delta DNA repair polymerase(s): - Answer - Beta or Epsilon Mitochondrial polymerase: - Answer - Gamma Molecular beacon design: - Answer - (R-sequence-Q) Which method uses visible light? - Answer - Pyrosequencing Lagging strand polymerase: - Answer - Alpha In NGS what is coverage referring to - Answer - # of times a base is read Which type of NGS is best used for small indels? - Answer - paired-end Number of SNPs in human genome? - Answer - 10^7 book answer 10^6 exam answer (closest answer available) FLT3 is involved in what disease? - Answer - AML RNA storage condition for 6 months? - Answer - -70C in Ethanol How long to keep obsolete document in lab according to CAP? - Answer - 2 years HIV viral load difference? - Answer - 0.5 log 10 Antibiotic resistance mechanism in MRSA? - Answer - altered drug target PBP2a mecA What is the effect of Irinotecan and UGT1A1? - Answer - Decrease drug metabolism What part of RNA increases half-life and stability? - Answer - G cap or 5' cap Formamide decreases Tm by: - Answer - altering H-bonds Common form of DNA is: - Answer - B form, Right-Handed Order to add to a PCR reaction - Answer - Master mix Patient sample Positive control Negative control Primer dimers are formed due to: - Answer - 3' Complementarity Virus affecting patients after kidney transplant is - Answer - BK Virus Warfarin is associated with? - Answer - VKORC1 and CYP2C9 Which is most specific PCR? - Answer - Nested PCR then Real Time PCR What is the proper GC content of primer? - Answer - 50-60% Which sample type to expect degraded DNA from? - Answer - FFPE Highest vancomycin resistance? - Answer - vanA HCV genotype 1 vs 2 : Which has a low viral load and best sustained response to drugs? - Answer - HCV Genotype 2 To avoid RNAse at what temp should you bake the glassware? - Answer - 250*C for 4 hours Aldehyde dehydrogenase question - Answer - Alcohol dependence Metabolizing alcohol