TSI Agar Slant Questions and Answers 2023

TSI Agar Slant Questions and Answers 2023 TSI stands for Triple Sugar Iron If peptone is metabolized then what is made Ammonia What are the 3 carbohydrates present for TSI Glucose Sucrose Lactose Glucose is a monosaccharide Sucrose and Lactose are disaccharides Monosaccharides differ from disaccharides by using less energy to be broken down Ph indicator that is used is Phenol Red The color of the agar if positive for an acid is Yellow The color of the agar if negative for an acid is Deep Red What are the 2 indicators for H2S Ferric Salt and Sodium Thinsulfate What color indicates H2S Black Incubation time is 18-24 hours at 35-37 C The top of the tube is cosidered to be aerobic conditions The bottom of the tube is considered anaerobic conditions The Bottom of the tube has no ________ and tends to produce more ______ that is more _______. oxygen acid stable How is this medium innoculated the top of the tube is zig zag streaked while the butt is stabbed Glucose fermenter only is inidcated by Red slant and yellow butt - alkaline top and acid bottom (glucose only) What happens after 8-10 hours lapse glucose is used up so peptone is starting to be used (glucose only) Slant turns red after 8-10 hours because there is so much ammonia being produced making it alkaline (glucose only) Could be part of the Enterobacteriaciae because it ferments glucose (glucose only) k/Ag indicates Red slant and yellow butt that is split (glucose only) k/A H2S indicates Red slant and black butt (glucose onbly) k/A H2S ferements glucose to produce acid and hydrogen sulfide, there has to be acid to have the H2S (glucose only) k/Ag H2S indicates red slant and black butt that is split Glucose and Lactose / Sucrose or both indicated by Yellow slant and yellow butt - acid is produced on both Glucose is being fermented as well as Sucrose and/or Lactose (glucose and S/l) After 8-10 hours and all the glucose is used then sucrose and lactose are being fermented (glucose and s/l) A/Ag indicates yellow slant and yellow butt with splitting or cracking (glucose and s/l) A/A h2s indicates Yellow slant and black butt (glucose and s/l) A/Ag h2s indicates yellow slant and black butt with splitting or cracking Glucose non fermenter is indicated by deep red slant and butt If test indicates that it is a glucose non fermenter then there was no reaction with the glucose