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Summary and notes on the chapter “Amino acid analysis and sequencing” of (Concepts of Protein technology and applications: Partim Boonen)

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57 pages
2022/2023 Written for academic year
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Universiteit Antwerpen (UA)
Course
Concepts Of Protein Technology And Applications (2050FBDBMW)
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This summary includes the summary and notes on the chapter “Amino acid analysis and sequencing” from the part of Concepts of Protein technology and applications taught by professor Boonen. A separate document is uploaded for the part taught by professor Van Ostade. Both parts need to be known for the exam!!!! The course Concepts of Protein technology and applications () is the new version of the old course called proteomics. The summary covers both parts of Prof. Van Ostade and Prof. Boonen and it contains everything you need to pass the exam (Achieved result = 18/20).

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Amino acid analysis

Receptor Biology Lab
University of Antwerp
Prof. Stuart Maudsley

, AA analyses and AA sequencing
are two different techniques

AA analysis – methodology used to determine the
AA composition or content of proteins,
peptides and pharmaceutical preparations


AA sequencing – identifies the exact primary
AA sequence of a protein/peptide

,What can Amino Acid Analysis (AAA) do for you ?
Can give you an exact knowledge of protein AA quantities =
required for further protein chemistry applications.

Allows measurement of relative amino acid composition of proteins and
complex protein mixtures

Facilitates measurement of free amino acids in media/fluids

Estimate amounts of contaminating protein in DNA and other non-protein
samples

Very accurate quantification: E.g. isotopically labeled internal standards
used for absolute quantification in LC-MS are calibrated by AAA

, What can Amino Acid Analysis (AAA) do for you ?
Facilitates measurement of free amino acids in media/fluids, e.g. in PKU

PKU = phenylketonuria, a genetic metabolic disease
During development PKU children cannot ingest Phe
(potentially life-threatening). Thus detection of Phe in
food/drugs is vital


normal


PKU
Phenylalanine
hydroxylase
deficiency

Document information

Study
Biomedische Wetenschappen
Uploaded on
February 13, 2023
Number of pages
57
Written in
2022/2023
Type
Class notes
Professor(s)
Kurt boonen
Contains
Aa_sequencing (part kurt boonen, amino acid analysis and sequencing, concepts of protein technology and applications)
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