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BIOLOGICAL 101 MICROBIOLOGY_LAB01_ASSESSMENT_PACKAGE.

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BIOLOGICAL 101 MICROBIOLOGY_LAB01_ASSESSMENT_PACKAGE. Exercise 1 Questions 1. What patterns do you observe based on the information in Table 4? The closer the pH is too neutral (7pH) the higher the number of colonies that are present. 2. Develop a hypothesis relating to the pH level of the culture media and the number of microbial colonies observed in each culture. The number of colonies increase when pH scale is closer to neutral. 3. What would your experimental approach be to test this hypothesis? Analyse the number of colonies in different pH levels. 4. What would be the independent and dependent variables? IV: pH of the solution DV: Number of colonies 5. What would be your control? H2O will be the control group 6. What type of graph would be appropriate for this data set? Why? A bell curve to show the distribution of colonies based on the ph. 7. Graph the data from Table 4. 8. Interpret the data from the graph you made in Question 7. The farther from neutral (7 pH) the less colonies that will appear. Exercise 2 Questions 1. Fresh-baked bread develops mold more quickly than bread bought from the store. Determine if the observation is qualitative or quantitative. • Quantitative Write a hypothesis and a null hypothesis. • The less preservatives in bread will mold more quickly The less preservatives in bread will not mold more quickly What would be your experimental approach? • Set two breads (fresh and store bought) in open air and monitor them on a set time table (example: every 2 hours). variables? • DV: rate of mold. IV: amount of preservatives. What are your controls - both positive and negative? • amount of light Temperature of surrounding Number of germs and toxins in the air. How will you collect your data? • Monitor the breads until mold appears on one. Then observe until the other bread appears and compare rates. Then continue to analyses both breads amount of mold. What are the dependent and independent How will you present your data (e.g., chart, graph)? • Graph of the two different breads by amount of mold as X and time it takes for the mold to develop. How will you analyze your data? • Observe the both data and analyze which took less time to start molding and the rate of molding. 2. Sally comes to work sick; two days later, three of her coworkers are also sick. Determine if the observation is qualitative or quantitative. • quantitative Write a hypothesis and a null hypothesis. • The more individuals that interaction with a sick individual, the more likely individuals will get sick. The more individuals that interaction with a sick individual, the more likely individuals will not get sick. What would be your experimental approach? • To take many individuals (ex. 5 healthy people) and interact for a set amount of time with an infected individual and see if they develop an infection. What are the dependent and independent variables? • DV: The amount sick IV: The amount of individuals What are your controls - both positive and negative? • Amount of time spent with infected The environment The infection The health history of the individuals Other exposers the healthy individuals have during the day How will you collect your data? • Will check the healthy individuals in a few days to see if they were infected. How will you present your data (e.g., chart, graph)? • a graph How will you analyze your data? • by checking how many are affected by the disease will tell how infectious the illness is. 3. You accidentally left a carton of milk on the counter all night, and you notice that the milk tastes worse than it usually does when it is stored in the refrigerator.

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