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BIOS 390 Week 6 LAB: Isolate Plasmid DNA (GRADED)

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Once a plasmid has … introduced into competent bacterial cells and the cells have grown into colonies on a medium selective for cells containing the plasmid, the next step is to perform a miniprep o f the plasmid DNA in preparation for DNA sequencing. The three steps are: 1) growing cells in liquid culture; 2) purifying the plasmid DNA from the culture; and 3) performing a restriction digest on the purified DNA to determine whether the DNA insert in the plasmid is the … size. To start the liquid culture, cells from an isolated bacterial colony are … in selective medium (nutrient broth with an antibiotic to which the plasmid provides resistance). ……….. document continue Show Less

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Institution
Western Illinois University
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BIOS 390









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Institution
Western Illinois University
Course
BIOS 390

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Uploaded on
October 20, 2022
Number of pages
7
Written in
2022/2023
Type
CASE
Professor(s)
Proff jj
Grade
A+

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  • bios 390 week 6 lab isolate plasmid dna graded
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