BIOD 171 Essential Microbiology Lab9 exam.

BIOD 171 Essential Microbiology Lab9 exam. Question 1 6 / 6 pts Using the observations made and recorded from PL09 Unknown Pathogen, complete the results sections accordingly. Given Culture A and Culture B were accidentally mixed, what observations were made from the Gram stain (shown below)? Your Answer: some of the bacteria are Gram positive which we can see in purple clusters , some other bacteria are Gram negative that they are pink and their shape are rod Gram-positive cocci (spherical) clusters and Gram-negative bacillus (rods) were observed. This study source was downloaded by from CourseH on :23:19 GMT -05:00 Question 2 2 / 6 pts Using the observations made and recorded from PL09 Unknown Pathogen, complete the results sections accordingly. Given the results from a MacConkey agar plate shown below, what is the shape of Culture A? Culture B? Your Answer: MacConkey is a selective media and only Gram negative bacteria grow on it Culture A- No growth Culture B- there is growth, culture B must be gram negative. MacConkey agar is selective for Gram-negative bacteria. Thus, Culture A must be Gram-positive as no growth was observed. Coupled to the results of the gram stain, Culture A (Gram-positive) must be the cocci. Culture B must be Gram-negative (growth observed) and this correlates to the bacillus or rod-shaped bacteria. what is the shape of Culture A? Culture B? Question 3 6 / 6 pts This study source was downloaded by from CourseH on :23:19 GMT -05:00 Using the observations made and recorded from PL09 Unknown Pathogen, complete the results sections accordingly. The hemolytic properties of both cultures were next examined by streaking each culture on a blood agar plate (shown below). What is the hemolytic status of Culture A? Culture B? Your Answer: it indicates beta hemolysis, because of the lyses in red blood cells the agar has change of color Culture B, it grows but it does not have any lyses quality Culture A exhibits beta hemolysis as defined by the region of clearing around the growing bacterial streak. Culture B exhibits gamma-hemolysis (non-hemolytic) as indicated by the white colonies growing on the still red agar. Question 4 6 / 6 pts Using the observations made and recorded from PL09 Unknown Pathogen, complete the results sections accordingly. Culture A and Culture B were assessed via the coagulase test as shown below. State your observations for each culture.