Class notes
intro to cryo-electron microscopy
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22-02-2022
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is a cryomicroscopy technique applied on samples cooled to cryogenic temperatures and embedded in an environment of vitreous water. An aqueous sample solution is applied to a grid-mesh and plunge-frozen in liquid ethane or a mixture of liquid ethane and propane.[2] While development of the technique began in the 1970s, recent advances in detector technology and software algorithms have allowed for the determination of biomolecular structures at near-atomic resolution.[3] This has attracted wide attention to the approach as an alternative to X-ray crystallography or NMR spectroscopy for macromolecular structure determination without the need for crystallization.
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Institution
Stanford University
Course
Cryo-EM
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Written for
- Institution
-
Stanford University
- Course
-
Cryo-EM
Document information
- Uploaded on
- February 22, 2022
- Number of pages
- 69
- Written in
- 2020/2021
- Type
- Class notes
- Professor(s)
- Ron dror
- Contains
- All classes
Subjects
- electron microscopy
- cryo em
-
cryogenic electron microscopy
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