Written by students who passed Immediately available after payment Read online or as PDF Wrong document? Swap it for free 4.6 TrustPilot
logo-home
Previously searched by you
Previously searched by you
logo
  • Toulouse III - Université Paul Sabatier
  • Science De La Vie
  • Biologie cellulaire
To add items to your wishlist, you must be logged in
Other

Techniques cellulaires

Rating
-
Sold
-
Pages
2
Uploaded on
06-01-2022
Written in
2021/2022

fiche de révision

Institution
Toulouse III - Université Paul Sabatier
Course
Biologie cellulaire

Content preview

Fractionnement cellulaire


Centrifugation differentielle
Le principe de fractio!ement cellulaire par centrifugation differentielle consiste a separer les organites selon leur ma!e. Elle permet
l'obtention d'un culot qui se depose au fond du tube et d'obtenir un surnagent. Cela permet de recuperer les fractions.

Les etapes experimentales sont :
- Collecte des cellules
- Homogeneisation a l'aide d'un Potter
- Centrifugation ba"e vite"e pour eliminer les noyaux, les cellules non ca"es
- Recuperation du surnagent




Centrifugation sur gradient de densite

On utilise la force centrifuge pour separer les organites d'un homogenat cellulaire les uns des autres selon leur difference de densite.

Les etapes experimentales sont :
- Collecte de cellules
- Homogeneisation a l'aide d'un Potter
- Centrifugation ba"e vite"e pour eliminer les noyaux, les cellules non ca"es
- Recuperation du surnageant et depot sur un gradient de sucrose
- Centrifugation
- Prelevement par fractions a partir du fond du tube




Western Blot
Le Western Blot permet de detecter la presence d'une proteine d'interet dans l'echantillon



SDS PAGE
Le SDS PAGE permet de separer les proteines en fonction de leur poids moleculaire.
Report Copyright Violation

Written for

Institution
Toulouse III - Université Paul Sabatier
Study
Science De La Vie
Course
Biologie cellulaire

Document information

Uploaded on
January 6, 2022
Number of pages
2
Written in
2021/2022
Type
OTHER
Person
Unknown

Subjects

  • biologie cellulaire
  • biologie
  • l2
$8.23
Get access to the full document:
Wrong document? Swap it for free Within 14 days of purchase and before downloading, you can choose a different document. You can simply spend the amount again.
Written by students who passed
Immediately available after payment
Read online or as PDF
Get to know the seller
Seller avatar
Ori2002

Get to know the seller

Seller avatar
Ori2002 Toulouse III - Université Paul Sabatier
View profile
Follow You need to be logged in order to follow users or courses
Sold
1
Member since
4 year
Number of followers
1
Documents
7
Last sold
4 year ago

0.0

0 reviews

5
0
4
0
3
0
2
0
1
0

Why students choose Stuvia

Created by fellow students, verified by reviews

Quality you can trust: written by students who passed their tests and reviewed by others who've used these notes.

Didn't get what you expected? Choose another document

No worries! You can instantly pick a different document that better fits what you're looking for.

Pay as you like, start learning right away

No subscription, no commitments. Pay the way you're used to via credit card and download your PDF document instantly.

Student with book image

“Bought, downloaded, and aced it. It really can be that simple.”

Alisha Student

Working on your references?

Create accurate citations in APA, MLA and Harvard with our free citation generator.

Learn more Create citation
Working on your references?

Frequently asked questions