BIOT643 Quiz1
Due in
Your assignment folder before midnight Sunday
For multiple choice questions (3 points each) highlight the correct answer, NOTE some
questions may have more than one possible answer.
For short answer questions, provide specific answers in your own words..
1. Which of the following primers shown below will hybridize with a DNA containing the
sequence (5')AGATCCAGA(3')?
A) (5')CTCATTGAG(3')
B) (5')GACCAGTCT(3')
C) (5')GAGTCAACT(3')
D) (5')TCTGACCAG(3')
E) (5')TCTGGATCT(3')
2. Which of the following separates molecules by movement due to size and electrical
charge?
A) restriction enzymes
B) gene cloning
C) DNA ligase
D) gel electrophoresis
E) reverse transcriptase
3. Dideoxyribonucleotide chain-termination is a method of
A) cloning DNA.
B) sequencing DNA.
C) digesting DNA.
D) synthesizing DNA.
E) separating DNA fragments.
4. After a PCR run using a new set of primers your gel shows no amplification bands at all.
What are some possible parameters with your reaction which might contribute to a non-
successful amplification?
A) Low annealing temperature
B) High annealing temperature
Due in
Your assignment folder before midnight Sunday
For multiple choice questions (3 points each) highlight the correct answer, NOTE some
questions may have more than one possible answer.
For short answer questions, provide specific answers in your own words..
1. Which of the following primers shown below will hybridize with a DNA containing the
sequence (5')AGATCCAGA(3')?
A) (5')CTCATTGAG(3')
B) (5')GACCAGTCT(3')
C) (5')GAGTCAACT(3')
D) (5')TCTGACCAG(3')
E) (5')TCTGGATCT(3')
2. Which of the following separates molecules by movement due to size and electrical
charge?
A) restriction enzymes
B) gene cloning
C) DNA ligase
D) gel electrophoresis
E) reverse transcriptase
3. Dideoxyribonucleotide chain-termination is a method of
A) cloning DNA.
B) sequencing DNA.
C) digesting DNA.
D) synthesizing DNA.
E) separating DNA fragments.
4. After a PCR run using a new set of primers your gel shows no amplification bands at all.
What are some possible parameters with your reaction which might contribute to a non-
successful amplification?
A) Low annealing temperature
B) High annealing temperature
