BIOT643 Quiz 2 (60 points)
Please Highlight the best answer(s)
(4 points each unless noted otherwise)
Bacteria protect their own DNA sequences from digestion by doing which one of the following
to the recognition sequence:
1. Phosphorylation
2. Glycosylation
3. Methylation
4. Deletion
5. Insertion
Type II restriction enzyme will NOT recognize which one of the following sequences:
5'–GTCATA- not a palindrome
3'–CAGTAT-
5'–GAATTC-
3'–CTTAAG-
5'–GATATC-
3'–CTATAG-
5'–GGATCC-
3'–CCTAGG-
Differential Display Analysis uses the following primer sets:
1. Two sequence specific primers
2. Two random primers
3. One random and one specific poly (A) primer
4. One poly (A) primer
Please Highlight the best answer(s)
(4 points each unless noted otherwise)
Bacteria protect their own DNA sequences from digestion by doing which one of the following
to the recognition sequence:
1. Phosphorylation
2. Glycosylation
3. Methylation
4. Deletion
5. Insertion
Type II restriction enzyme will NOT recognize which one of the following sequences:
5'–GTCATA- not a palindrome
3'–CAGTAT-
5'–GAATTC-
3'–CTTAAG-
5'–GATATC-
3'–CTATAG-
5'–GGATCC-
3'–CCTAGG-
Differential Display Analysis uses the following primer sets:
1. Two sequence specific primers
2. Two random primers
3. One random and one specific poly (A) primer
4. One poly (A) primer
