BIOT 643 --
Quiz 5 (60 points)
Please highlight the best answer
1. Which one of the following is NOT a characteristic of DNA Vaccine? (5 points)
a. It is produced in vitro
b. It can be produced at a low cost
c. It is easy to purify
d. It works with the DNA being converted to a protein
2. Which of the following is not true for polyclonal antibodies? (5 points)
a. They contain a mixture of antibodies
b. They are raised against purified proteins
c. They can be used to detect targets that differ in only a single antigenic
determinant
d. The amounts of antibodies in a polyclonal preparation may vary from one batch to
another
3. The underlying principle of gene therapy is (5 points)
a. Replacing a defective copy of a gene with a functional copy
b. Silencing a gene so it stops producing mRNA
c. Making a mutation in an existing gene
d. inserting a promoter to produce large amounts of a protein
4. A new viral pathogen has emerged, and you have been asked to develop an ELISA-based
detection assay to the intact virions. In order to ensure that a mutation in a single surface
protein does not render your assay obsolete, which of the following should you use?
a. Fluorescence in situ hybridization (FISH) (5 points)
b. Molecular beacons
c. Monoclonal antibodies
d. Polyclonal antibodies
e. Subunit vaccine
Quiz 5 (60 points)
Please highlight the best answer
1. Which one of the following is NOT a characteristic of DNA Vaccine? (5 points)
a. It is produced in vitro
b. It can be produced at a low cost
c. It is easy to purify
d. It works with the DNA being converted to a protein
2. Which of the following is not true for polyclonal antibodies? (5 points)
a. They contain a mixture of antibodies
b. They are raised against purified proteins
c. They can be used to detect targets that differ in only a single antigenic
determinant
d. The amounts of antibodies in a polyclonal preparation may vary from one batch to
another
3. The underlying principle of gene therapy is (5 points)
a. Replacing a defective copy of a gene with a functional copy
b. Silencing a gene so it stops producing mRNA
c. Making a mutation in an existing gene
d. inserting a promoter to produce large amounts of a protein
4. A new viral pathogen has emerged, and you have been asked to develop an ELISA-based
detection assay to the intact virions. In order to ensure that a mutation in a single surface
protein does not render your assay obsolete, which of the following should you use?
a. Fluorescence in situ hybridization (FISH) (5 points)
b. Molecular beacons
c. Monoclonal antibodies
d. Polyclonal antibodies
e. Subunit vaccine
