Pouyan Nikookar
BIOT643-UMGC
Midterm Exam (150 points)
Due in your assignment folder by 11:59 PM EST Sunday.
Based on honor code - Answers provided must be in your own words. You may use course
material and other scientific references. Please document any source you use NOT from this
semester’s BIOT 643
Part I: Multiple choice Questions: Highlight the most appropriate answers (more than one
answer may be correct).
(4 points each).
1.The DNA fragments making up a genomic library are generally contained in:
A) recombinant plasmids of bacteria.
B) recombinant viral RNA.
C) individual wells.
D) DNA-RNA hybrids
E) radioactive eukaryotic cells
2. RNAi can be used for one of the following purposes.
a) To disrupt a DNA termination sequence
b) To clone a particular gene
c) To investigate the function of a gene
d) To produce a new gene
3. Which of the following primer sequences has the lowest Tm?
A) AAACCCCCCTTTGGGAAA
B) GGGAAACCCTTTCCCGGG
C) AAAAAAGGGGGGTTTTTT
D) GGGTTTAAATTTAAACCC
E) AAATTTAAATTTAAATTT
4. Bacteria are unsuitable for expression of some eukaryotic proteins, because:
a) They lack a nuclear membrane
b) They lack introns
c) They don’t perform all post-translational modifications
d) They produce unsuitably stable proteins
, 5. To successfully express a protein from a eukaryotic expression vector, the following is
NOT required.
a) Selectable marker
b) Ribosome binding site
c) Strong eukaryotic promoter
d) Termination sequence
6. Which is a mechanism cells have evolved for regulating gene expression?
a) Upregulation of promotor activity
b) Downregulation of promotor activity
c) Relative promotor strength
d) All are mechanisms used by cells to regulate gene expression
e) None are mechanisms used by cells to regulate gene expression
7. Which of the following methods is NOT commonly used for high throughput gene expression
analysis?
a) Differential Display analysis
b) Subtractive Hybridization
c) DNA microarray
d) Northern blot hybridization
8. A PCR reaction where multiple amplicons are produced within the same tube is an example of
what PCR variation?
A) Multiplexed PCR
B) Nested PCR
C) Inverse PCR
D) Colony PCR
9. The principle behind chain-termination sequencing relies on which of the following being different
than in regular dNTPs?
A) 1’ carbon: uracil is bound instead of thymine
B) 2’ carbon: –H is bound instead of –OH
C) 3’ carbon: –H is bound instead of –OH
BIOT643-UMGC
Midterm Exam (150 points)
Due in your assignment folder by 11:59 PM EST Sunday.
Based on honor code - Answers provided must be in your own words. You may use course
material and other scientific references. Please document any source you use NOT from this
semester’s BIOT 643
Part I: Multiple choice Questions: Highlight the most appropriate answers (more than one
answer may be correct).
(4 points each).
1.The DNA fragments making up a genomic library are generally contained in:
A) recombinant plasmids of bacteria.
B) recombinant viral RNA.
C) individual wells.
D) DNA-RNA hybrids
E) radioactive eukaryotic cells
2. RNAi can be used for one of the following purposes.
a) To disrupt a DNA termination sequence
b) To clone a particular gene
c) To investigate the function of a gene
d) To produce a new gene
3. Which of the following primer sequences has the lowest Tm?
A) AAACCCCCCTTTGGGAAA
B) GGGAAACCCTTTCCCGGG
C) AAAAAAGGGGGGTTTTTT
D) GGGTTTAAATTTAAACCC
E) AAATTTAAATTTAAATTT
4. Bacteria are unsuitable for expression of some eukaryotic proteins, because:
a) They lack a nuclear membrane
b) They lack introns
c) They don’t perform all post-translational modifications
d) They produce unsuitably stable proteins
, 5. To successfully express a protein from a eukaryotic expression vector, the following is
NOT required.
a) Selectable marker
b) Ribosome binding site
c) Strong eukaryotic promoter
d) Termination sequence
6. Which is a mechanism cells have evolved for regulating gene expression?
a) Upregulation of promotor activity
b) Downregulation of promotor activity
c) Relative promotor strength
d) All are mechanisms used by cells to regulate gene expression
e) None are mechanisms used by cells to regulate gene expression
7. Which of the following methods is NOT commonly used for high throughput gene expression
analysis?
a) Differential Display analysis
b) Subtractive Hybridization
c) DNA microarray
d) Northern blot hybridization
8. A PCR reaction where multiple amplicons are produced within the same tube is an example of
what PCR variation?
A) Multiplexed PCR
B) Nested PCR
C) Inverse PCR
D) Colony PCR
9. The principle behind chain-termination sequencing relies on which of the following being different
than in regular dNTPs?
A) 1’ carbon: uracil is bound instead of thymine
B) 2’ carbon: –H is bound instead of –OH
C) 3’ carbon: –H is bound instead of –OH
