Section I: Multiple Choice Questions (Please highlight the best answer(s), 4
points each, unles otherwise noted – total 60 points)
1. An antibody is a molecule which:
a. Is produced by Memory B cells
b. Induces the production of antigens
c. Has a heavy and light chain
d. Is a fluorescently labeled reporter
2. Match a technique in Column A to the most appropriate application in Column B
(draw a line using draw tab in Word) or indicate by reordering column B to match
A. 8 pts.
________A___________ _____B________________________________
a) Immunofluorescence 1) Amplify a specific sequence of DNA
b) qRT-PCR 2) Examine the amino acid sequence of a protein
c) Mass Spectrometry 3) Quantify protein expression levels
d) Western blotting 4) Quantify a single gene’s expression level
e) ChIP 5) Identify protein-protein interactions
f) FRET 6) Determine where a protein is expressed
g) RNAseq 7) Identify protein-DNA interactions
h) PCR 8) Analyze global gene expression levels
, 3. DNA coated gold particles fired into the cell with a helium pressurized gun is best
classified as the following transfection method:
a. Electroporation
b. Lipofection
c. Biolistics
d. Transduction
4. Which one of the following methods is primarily used for protein identification and
structure determination?
a. Mass spectrometry
b. Microarray technology
c. Immunoprecipitation
d. Western Blot analysis
5. Which of the following promoters would you use to express a bacterial gene in a
mammal?
a. mammalian
b. bacterial
c. both (a) and (b)
d. neither (a) nor (b)
6. A vaccination method which provides immunity without requiring the injection of
an antigen is
a. Whole killed cell vaccine
b. DNA vaccine
c. Viral component vaccine
d. Peptide subunit vaccine
7. Which of the following are characteristics of all eukaryotic expression systems?
a. Secretion of factors to the medium
b. Rapid cell growth
c. Proper folding
d. Proper post-translational modifications
8. When transforming a plant using the Agrobacterium tumefaciens plasmid system,
what specifically is transferred into the plant cell.
points each, unles otherwise noted – total 60 points)
1. An antibody is a molecule which:
a. Is produced by Memory B cells
b. Induces the production of antigens
c. Has a heavy and light chain
d. Is a fluorescently labeled reporter
2. Match a technique in Column A to the most appropriate application in Column B
(draw a line using draw tab in Word) or indicate by reordering column B to match
A. 8 pts.
________A___________ _____B________________________________
a) Immunofluorescence 1) Amplify a specific sequence of DNA
b) qRT-PCR 2) Examine the amino acid sequence of a protein
c) Mass Spectrometry 3) Quantify protein expression levels
d) Western blotting 4) Quantify a single gene’s expression level
e) ChIP 5) Identify protein-protein interactions
f) FRET 6) Determine where a protein is expressed
g) RNAseq 7) Identify protein-DNA interactions
h) PCR 8) Analyze global gene expression levels
, 3. DNA coated gold particles fired into the cell with a helium pressurized gun is best
classified as the following transfection method:
a. Electroporation
b. Lipofection
c. Biolistics
d. Transduction
4. Which one of the following methods is primarily used for protein identification and
structure determination?
a. Mass spectrometry
b. Microarray technology
c. Immunoprecipitation
d. Western Blot analysis
5. Which of the following promoters would you use to express a bacterial gene in a
mammal?
a. mammalian
b. bacterial
c. both (a) and (b)
d. neither (a) nor (b)
6. A vaccination method which provides immunity without requiring the injection of
an antigen is
a. Whole killed cell vaccine
b. DNA vaccine
c. Viral component vaccine
d. Peptide subunit vaccine
7. Which of the following are characteristics of all eukaryotic expression systems?
a. Secretion of factors to the medium
b. Rapid cell growth
c. Proper folding
d. Proper post-translational modifications
8. When transforming a plant using the Agrobacterium tumefaciens plasmid system,
what specifically is transferred into the plant cell.
