1|Page
(MB) ASCP Exam 2026|Practice
Questions And Answers 2026
In what part of a qPCR Amplification Plot do you take your measurement? -
correct-answer - Exponential phase
All of the following are liquid tumors, except:
Mantle cell lymphoma
Ewing sarcoma
Burkitt's lymphoma
Acute promyelocytic leukemia - correct-answer - Ewing sarcoma
A technologist uses the spectrophotometer to quantify the amount of DNA
extracted from a blood specimen diluted 1:30. The absorbance reading at 260 nm
,2|Page
was found to be 2.545. If the absorbance at 280 nm gave a reading of 1.406, and
if the the DNA extract was re-suspended in 0.800 mL of EDTA solution, the DNA
yield is: - correct-answer - (2.545 * 50ng/mcl)= 127.25
(127.25ng/mcl * 0.30) = 38.175ng/mcl
38.175ng/mcl ( 0.800mL1000) =
3054 micrograms
According to the wobble hypothesis, a U in the 5' position of the anit-codon can
pair with:
U or C
A or G
A, C or G
Only A - correct-answer - A or G
,3|Page
This polymerase acts on DNA and produces Messenger RNA: - correct-answer -
RNA Pol II
What assay amplifies the probe signal and the probe RNA concentration increases
if the target to be detected is present?
Branched DNA
TMA
PCR
NASBA
LCR
Qβ replicase - correct-answer - Qβ replicase
This restriction enzyme "digests and adds a methyl group from Adenine":
Type I
Type II
Type III
DNase I - correct-answer - Type III
, 4|Page
What increases the half-life of mRNA?
5' Methyl Cap
3' Methyl Cap
Poly-A tail
5' Methionine Cap - correct-answer - 5' Methyl Cap
A parent has an autosomal dominant disorder. What percent chance does this
parent have to pass down this affected gene to his/her child?
0%
25%
50%
75%
100% - correct-answer - 50%
(MB) ASCP Exam 2026|Practice
Questions And Answers 2026
In what part of a qPCR Amplification Plot do you take your measurement? -
correct-answer - Exponential phase
All of the following are liquid tumors, except:
Mantle cell lymphoma
Ewing sarcoma
Burkitt's lymphoma
Acute promyelocytic leukemia - correct-answer - Ewing sarcoma
A technologist uses the spectrophotometer to quantify the amount of DNA
extracted from a blood specimen diluted 1:30. The absorbance reading at 260 nm
,2|Page
was found to be 2.545. If the absorbance at 280 nm gave a reading of 1.406, and
if the the DNA extract was re-suspended in 0.800 mL of EDTA solution, the DNA
yield is: - correct-answer - (2.545 * 50ng/mcl)= 127.25
(127.25ng/mcl * 0.30) = 38.175ng/mcl
38.175ng/mcl ( 0.800mL1000) =
3054 micrograms
According to the wobble hypothesis, a U in the 5' position of the anit-codon can
pair with:
U or C
A or G
A, C or G
Only A - correct-answer - A or G
,3|Page
This polymerase acts on DNA and produces Messenger RNA: - correct-answer -
RNA Pol II
What assay amplifies the probe signal and the probe RNA concentration increases
if the target to be detected is present?
Branched DNA
TMA
PCR
NASBA
LCR
Qβ replicase - correct-answer - Qβ replicase
This restriction enzyme "digests and adds a methyl group from Adenine":
Type I
Type II
Type III
DNase I - correct-answer - Type III
, 4|Page
What increases the half-life of mRNA?
5' Methyl Cap
3' Methyl Cap
Poly-A tail
5' Methionine Cap - correct-answer - 5' Methyl Cap
A parent has an autosomal dominant disorder. What percent chance does this
parent have to pass down this affected gene to his/her child?
0%
25%
50%
75%
100% - correct-answer - 50%
