GMS 6121 Exam 1 Complete Study
Guide (Questions and Verified
Answers) Already Graded A+ |
Brand New Version
1. Virus genes code for proteins that are important for: - ANSWER a. Virus
genome replication
b. Formation of the capsid
c. Virion assembly
d. Evasion of intracellular defense system
e. Evasion of the extracellular immune response
2. The number of plaques detected in one well of a plaque assay is a reflection
of both the amount of virus in the sample AND the amount of___________.
A. Lipid in sample
B. RBC in sample
C. Dilution of sample
D. Sample agglutination
E. Immune cells in the sample. - ANSWER C. Dilution of sample
3. By far the most common assay used in clinical virology labs to detect
specific virus infections is the:
A. Serology Assay
B. Transformation assay
, C. PCR Assay
D. ELISA Assay
E. Hem agglutination assay - ANSWER C. PCR Assay
4. True or False: the receptor binding domain of all picornavirus resides in the
protruding domains of the virion surface, also referred to as the plateaus. -
ANSWER False, it occurs in the canyon
5. What is nucleic acid detection? - ANSWER real-time qPCR or RT-qPCR
which can be used to quantify the number of copies of virus genomes in a
sample
qPCR = measuring DNA virus genomes
RT-qPCR = measuring RNA virus genomes
IgG vs IgM - ANSWER IgM is detectable within 1 week of infection.
IgG produced approximately 2 weeks after an infection, may be detectable for
months to years after an infection.
6. Majority of viral infections are: - ANSWER subclinical
7. Hemagglutinin binds to sialic acid, ________ cleaves the receptor. -
ANSWER NEURAMINIDASE
8. Tamiflu inhibits ______, blocking any new virion release. - ANSWER
NEURAMINIDASE
,9. Which layer of skin is highly vascularized and can allow infections to
disseminate?
A. Dermis
B. Epidermis - ANSWER Dermis (and sub-dermal)
10.Respiratory Tract is the most common route of viral entry: What are the 3
common barriers to infection? - ANSWER Barriers to infection:
Mechanical (mucous traps viruses, ciliary action propels viruses out of lungs,
sneezing & coughing)
Cellular (macrophages in alveoli)
Humoral (IgA)
11.General stages of virus infection - ANSWER 1) Adsorption
2) Entry
3) Uncoating
4) Early genes
5) Replication
6) Late genes
7) Assembly
8) Packaging
9) Release
12.Simplest icosahedral are formed by _____ identical proteins, in a _____-
protein complex, to form _____ faces. - ANSWER 60, 3, 20
, 13.How many groups in Baltimore classification system and how are they
classified? - ANSWER VII, takes into account the virus genome structure,
and whether or not the virus uses reverse transcriptase.
14.RNA has which polymerase encoded into it's gene sequence - ANSWER
RDRP: RNA dependent RNA polymerase
15.In (-)ssRNA where is the RDRP stored - ANSWER Within it's capsid
16.An eclipse occurs at what part of viral growth? - ANSWER Directly
following replication before assembly
17.Cytopathic effect (CPE) Includes which of the following:
A. Cell lysis
B. Rounding (or other morphological changes)
C. Syncytial
D. Inclusion bodies
E. All of the Above - ANSWER E
18.Hemadsorption=attachment of an infected cell to RBCs. This is indicative of
glycoprotein/hemaglutinin expression on the host cell surface. What disease
would hemadsorption be indicative of? - ANSWER Influenza
Immortalization, Colony formation, Tumor formation in immunocompromised
mice, Loss of anchorage dependence, and Loss of contact inhibition are all
characteristics of? - ANSWER Transformation
______ detection results in the presence of viral particles, viral proteins, and virus
nucleic acid. _________ detection uses antibodies for diagnosis. - ANSWER
Direct; Indirect
Guide (Questions and Verified
Answers) Already Graded A+ |
Brand New Version
1. Virus genes code for proteins that are important for: - ANSWER a. Virus
genome replication
b. Formation of the capsid
c. Virion assembly
d. Evasion of intracellular defense system
e. Evasion of the extracellular immune response
2. The number of plaques detected in one well of a plaque assay is a reflection
of both the amount of virus in the sample AND the amount of___________.
A. Lipid in sample
B. RBC in sample
C. Dilution of sample
D. Sample agglutination
E. Immune cells in the sample. - ANSWER C. Dilution of sample
3. By far the most common assay used in clinical virology labs to detect
specific virus infections is the:
A. Serology Assay
B. Transformation assay
, C. PCR Assay
D. ELISA Assay
E. Hem agglutination assay - ANSWER C. PCR Assay
4. True or False: the receptor binding domain of all picornavirus resides in the
protruding domains of the virion surface, also referred to as the plateaus. -
ANSWER False, it occurs in the canyon
5. What is nucleic acid detection? - ANSWER real-time qPCR or RT-qPCR
which can be used to quantify the number of copies of virus genomes in a
sample
qPCR = measuring DNA virus genomes
RT-qPCR = measuring RNA virus genomes
IgG vs IgM - ANSWER IgM is detectable within 1 week of infection.
IgG produced approximately 2 weeks after an infection, may be detectable for
months to years after an infection.
6. Majority of viral infections are: - ANSWER subclinical
7. Hemagglutinin binds to sialic acid, ________ cleaves the receptor. -
ANSWER NEURAMINIDASE
8. Tamiflu inhibits ______, blocking any new virion release. - ANSWER
NEURAMINIDASE
,9. Which layer of skin is highly vascularized and can allow infections to
disseminate?
A. Dermis
B. Epidermis - ANSWER Dermis (and sub-dermal)
10.Respiratory Tract is the most common route of viral entry: What are the 3
common barriers to infection? - ANSWER Barriers to infection:
Mechanical (mucous traps viruses, ciliary action propels viruses out of lungs,
sneezing & coughing)
Cellular (macrophages in alveoli)
Humoral (IgA)
11.General stages of virus infection - ANSWER 1) Adsorption
2) Entry
3) Uncoating
4) Early genes
5) Replication
6) Late genes
7) Assembly
8) Packaging
9) Release
12.Simplest icosahedral are formed by _____ identical proteins, in a _____-
protein complex, to form _____ faces. - ANSWER 60, 3, 20
, 13.How many groups in Baltimore classification system and how are they
classified? - ANSWER VII, takes into account the virus genome structure,
and whether or not the virus uses reverse transcriptase.
14.RNA has which polymerase encoded into it's gene sequence - ANSWER
RDRP: RNA dependent RNA polymerase
15.In (-)ssRNA where is the RDRP stored - ANSWER Within it's capsid
16.An eclipse occurs at what part of viral growth? - ANSWER Directly
following replication before assembly
17.Cytopathic effect (CPE) Includes which of the following:
A. Cell lysis
B. Rounding (or other morphological changes)
C. Syncytial
D. Inclusion bodies
E. All of the Above - ANSWER E
18.Hemadsorption=attachment of an infected cell to RBCs. This is indicative of
glycoprotein/hemaglutinin expression on the host cell surface. What disease
would hemadsorption be indicative of? - ANSWER Influenza
Immortalization, Colony formation, Tumor formation in immunocompromised
mice, Loss of anchorage dependence, and Loss of contact inhibition are all
characteristics of? - ANSWER Transformation
______ detection results in the presence of viral particles, viral proteins, and virus
nucleic acid. _________ detection uses antibodies for diagnosis. - ANSWER
Direct; Indirect
