BIO101 ACTUAL TEST PAPER FULLY
SOLVED QUESTIONS AND CORRECT
ANSWERS
●● A.l
Answer: Which of the following describes DNA within a eukaryotic
cell? (Select all that apply) DNA is surrounded by two separate
phospholipid membranes within the cell. DNA is packaged around
proteins within the nucleus. The phosphate group of the nucleotides
within DNA have a negative charge.
●● UGG
Answer: In translation, what would be the correct tRNA anticodon for
the codon ACC?
●● uracil; cytosine
Answer: In protein synthesis, adenine pairs with and guanine pairs with
●● ATGGCTACG
Answer: Using the correct base pairing rules for DNA replication, what
would be the complementary strand for the strand TACCGATGC?
●● DNA replication
,Answer: is the process by which a parental chromosome is copied and
yields two new daughter chromosomes.
●● nucleotides
Answer: The sequence of in nucleic acids encodes a specific order of
amino acids for making a protein.
●● 1, 2
Answer: In order for the DNA samples to TRAVEL across the gel
accurately, it is important that (Select all that apply) the voltage is set
correctly on the chamber. the electrophoresis buffer is poured over the
agarose gel. the pH of the gel is correct. bromophenol blue dye is used.
All of the answer choices are correct.
●● False
Answer: A high voltage rate will cause the DNA fragments to move
slowly across the gel. True or False
●● True
Answer: The tape must be removed from the chamber tray prior to
running the DNA samples because the tape will prevent the buffer from
completely surrounding the gel. True or False
●● genotype
,Answer: In the gel electrophoresis lab, you determined the of three
different DNA samples.
●● False
Answer: The electrophoresis buffer is poured over the agarose gel
because it charges the DNA samples so they can travel more readily
across the chamber. True or False
●● True
Answer: Ethidium bromide is a dye that is used to stain the gel and
allows the DNA to be viewed under UV light. True or False
●● micropipet.
Answer: In gel electrophoresis, how are the DNA samples added to the
wells?
●● 1,2,3
Answer: Gel electrophoresis can be used to separate molecules by
(Select all that apply) length. size. charge. weight. number of atoms.
●● it facilitates homogenization.
Answer: Detergent is used in the DNA isolation process because
●● in one direction.
, Answer: In order to prevent the DNA from breaking, the glass rod
should be spun
●● True
Answer: In the DNA isolation process, cells are mixed with sodium
chloride (i.e. NaCI) because the sodium (Na) neutralizes the negative
charge of DNA. True or False
●● True
Answer: Ethanol cannot be added to the test tube too quickly because it
will break up the DNA precipitate. True or False
●● stabilize the DNA.
Answer: In the DNA isolation process, the filtrate and reagents are kept
in an ice bath because cold temperatures are required to
●● emulsify cellular and nuclear membranes.
Answer: In the DNA isolation process, detergent was used to
●● False
Answer: Homogenization is the process that breaks down large fat
globules into smaller fat globules. True or False
●● Biotechnology
SOLVED QUESTIONS AND CORRECT
ANSWERS
●● A.l
Answer: Which of the following describes DNA within a eukaryotic
cell? (Select all that apply) DNA is surrounded by two separate
phospholipid membranes within the cell. DNA is packaged around
proteins within the nucleus. The phosphate group of the nucleotides
within DNA have a negative charge.
●● UGG
Answer: In translation, what would be the correct tRNA anticodon for
the codon ACC?
●● uracil; cytosine
Answer: In protein synthesis, adenine pairs with and guanine pairs with
●● ATGGCTACG
Answer: Using the correct base pairing rules for DNA replication, what
would be the complementary strand for the strand TACCGATGC?
●● DNA replication
,Answer: is the process by which a parental chromosome is copied and
yields two new daughter chromosomes.
●● nucleotides
Answer: The sequence of in nucleic acids encodes a specific order of
amino acids for making a protein.
●● 1, 2
Answer: In order for the DNA samples to TRAVEL across the gel
accurately, it is important that (Select all that apply) the voltage is set
correctly on the chamber. the electrophoresis buffer is poured over the
agarose gel. the pH of the gel is correct. bromophenol blue dye is used.
All of the answer choices are correct.
●● False
Answer: A high voltage rate will cause the DNA fragments to move
slowly across the gel. True or False
●● True
Answer: The tape must be removed from the chamber tray prior to
running the DNA samples because the tape will prevent the buffer from
completely surrounding the gel. True or False
●● genotype
,Answer: In the gel electrophoresis lab, you determined the of three
different DNA samples.
●● False
Answer: The electrophoresis buffer is poured over the agarose gel
because it charges the DNA samples so they can travel more readily
across the chamber. True or False
●● True
Answer: Ethidium bromide is a dye that is used to stain the gel and
allows the DNA to be viewed under UV light. True or False
●● micropipet.
Answer: In gel electrophoresis, how are the DNA samples added to the
wells?
●● 1,2,3
Answer: Gel electrophoresis can be used to separate molecules by
(Select all that apply) length. size. charge. weight. number of atoms.
●● it facilitates homogenization.
Answer: Detergent is used in the DNA isolation process because
●● in one direction.
, Answer: In order to prevent the DNA from breaking, the glass rod
should be spun
●● True
Answer: In the DNA isolation process, cells are mixed with sodium
chloride (i.e. NaCI) because the sodium (Na) neutralizes the negative
charge of DNA. True or False
●● True
Answer: Ethanol cannot be added to the test tube too quickly because it
will break up the DNA precipitate. True or False
●● stabilize the DNA.
Answer: In the DNA isolation process, the filtrate and reagents are kept
in an ice bath because cold temperatures are required to
●● emulsify cellular and nuclear membranes.
Answer: In the DNA isolation process, detergent was used to
●● False
Answer: Homogenization is the process that breaks down large fat
globules into smaller fat globules. True or False
●● Biotechnology
